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采用静置贴壁细胞培养法，体外长期培养了胎儿、儿童、成人骨髓基质细胞，时间为６个月，可传至１０代，并观察了成纤维肌样细胞、内皮细胞和巨噬细胞；通过免疫细胞化学染色法，证明了１～３代的骨髓基质肌样细胞Ｖｉｅｍｅｎｔｉｎ为阳性，第Ⅷ因子阴性；采用流式细胞仪检测法，证明了儿童骨髓基质细胞的细胞表型为ＣＤ３３－和ＣＤ３４－、ＣＤ３８－、ＣＤ＋Ｗ９０，成人骨髓基质细胞为ＣＤ－３３、ＣＤ－３４、ＣＤ＋３８、ＣＤ＋Ｗ９０。 采用体外培养和扩增的脐血造血干细胞的半固体集落形成法，证明了基质细胞能长期维持脐血中的ＬＴＣ－ＩＣ的生存，若加入ＩＬ－６、ＩＬ－３、ＳＣＦ细胞因子的扩增体系，基质细胞对长期维持和扩增ＬＴＣ－ＩＣ的效果更为明显（Ｐ＜０．０１），比无基质细胞的对照组所形成的ＬＴＣ－ＩＣ产率高于２～４倍。表明骨髓基质细胞具有支持造血功能。
Adopting static adherent cell culture method, fetal, children and adult bone marrow stromal cells were cultured in vitro for 6 months, which could be transmitted to 10 passages and observed fibroblastoid cells, endothelial cells and macrophages. Immunocytochemical staining showed that Viementin of bone marrow stromal cells from 1 to 3 generations was negative and factor Ⅷ was negative. Flow cytometry was used to confirm that the cell phenotype of childhood bone marrow stromal cells was CD33- CD34-, CD38-, CD + W90, adult bone marrow stromal cells are CD-33, CD-34, CD + 38, CD + W90. Using semi-solid colony formation of cord blood hematopoietic stem cells cultured and expanded in vitro, it was proved that stromal cells could maintain the survival of LTC-IC in cord blood for a long time. If IL-6, IL-3 and SCF cytokines were added Increased system, stromal cells on the long-term maintenance and expansion of LTC-IC effect was more significant (P <0.01), compared with stromal cells control group formed LTC-IC yield higher than 2 to 4 times. Bone marrow stromal cells showed hematopoietic function.