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目的探讨维吾尔族妇女宫颈鳞癌组织中核黄素水平与核黄素转运基因2(riboflavin transporter 2gene,hRFT2)表达的关系及意义。方法收集维吾尔族妇女宫颈鳞癌患者的新鲜组织标本,采用酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)方法检测宫颈癌组织及相应癌旁组织中核黄素水平,同时应用实时荧光定量PCR(qRT-PCR)方法检测宫颈癌组织及相应癌旁组织中hRFT2mRNA的表达,分析核黄素水平与hRFT2mRNA表达的关系。采用免疫组织化学SP法检测50例宫颈鳞癌组织及相应癌旁组织中hRFT2蛋白的表达。结果宫颈鳞癌组织中核黄素水平(16.13±3.88)明显低于相应癌旁组织(19.92±4.11),差异有统计学意义(P<0.05)。hRFT2mRNA在宫颈鳞癌组织中的表达量(0.21±0.14)明显高于癌旁组织(0.16±0.12),差异有统计学意义(P<0.05);宫颈鳞癌组织中核黄素水平与核黄素转运基因的转录水平呈负相关(r=-2.025,P=0.039)。hRFT2蛋白在宫颈鳞癌组织中的表达明显高于癌旁组织,其阳性表达主要定位于细胞浆和或细胞膜,而正常宫颈组织中主要定位于细胞核(P<0.05)。尽管hRFT2蛋白表达水平在宫颈癌的不同分化程度以及有无淋巴结转移间存在相应变化,但表达差异无统计学意义(P>0.05)。仅与宫颈癌的临床分期密切相关(P<0.05)。结论维吾尔族妇女宫颈癌组织中hRFT2mRNA异常转录影响组织核黄素水平,其可能通过改变蛋白的活性或定位而影响其对核黄素的转运功能。
Objective To investigate the relationship between the expression of riboflavin transporter 2 (hRFT2) and the expression of riboflavin transporter 2 (hRFT2) in Uighur women with cervical squamous cell carcinoma. Methods Fresh tissue samples from Uigur women with cervical squamous cell carcinoma were collected. The levels of riboflavin in cervical cancer tissues and corresponding paracancerous tissues were detected by enzyme-linked immunosorbent assay (ELISA). Real-time quantitative PCR qRT-PCR) was used to detect the expression of hRFT2mRNA in cervical cancer tissues and corresponding paracancerous tissues. The relationship between the expression of hRFT2mRNA and riboflavin expression was analyzed. Immunohistochemical SP method was used to detect the expression of hRFT2 protein in 50 cases of cervical squamous cell carcinoma and corresponding paracancerous tissues. Results The level of riboflavin in cervical squamous cell carcinoma (16.13 ± 3.88) was significantly lower than that in adjacent noncancerous tissues (19.92 ± 4.11) (P <0.05). The expression of hRFT2 mRNA in cervical squamous cell carcinoma was significantly higher than that in adjacent non-cancerous tissues (0.21 ± 0.14 vs 0.16 ± 0.12, P <0.05) Transcriptional gene transcriptional levels were negatively correlated (r = -2.025, P = 0.039). The expression of hRFT2 protein in cervical squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues. The positive expression of hRFT2 mainly localized in the cytoplasm and the cell membrane, while the normal cervical tissues mainly located in the nucleus (P <0.05). Although hRFT2 protein expression levels in cervical cancer with different degrees of differentiation and lymph node metastasis there is a corresponding change, but the expression difference was not statistically significant (P> 0.05). Only with the clinical stage of cervical cancer is closely related (P <0.05). Conclusion Abnormal transcription of hRFT2 mRNA in Uighur women with cervical cancer affects the level of riboflavin, which may affect the function of riboflavin by changing the activity or localization of the protein.