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为了解北京市HIV-1亚型特点和流行特征,随机采集北京市2007年新确证HIV-1感染者的抗凝全血标本200份,分离血浆,提取病毒RNA,用套式聚合酶链式反应扩增病毒gag基因,并进行序列测定和亚型分析。系统进化分析确定北京市HIV-1流行毒株分别属于7个亚型和流行重组型,分别为A1亚型1份,B亚型35份,泰国B亚型19份,C亚型3份,CRF01_AE亚型49份,CRF07_BC亚型51份,CRF08_BC亚型3份。北京市己存在7种HIV-1亚型和流行重组型,应该加强北京市HIV-1亚型流行情况的监测。
In order to understand the characteristics and epidemiological characteristics of HIV-1 subtype in Beijing, 200 samples of anticoagulated whole blood of newly confirmed HIV-1 infected persons in Beijing were randomly collected from Beijing in 2007. The plasma was isolated and the viral RNA was extracted. The virus gag gene was amplified and sequenced and subtype analyzed. Phylogenetic analysis showed that the HIV-1 strains in Beijing belonged to 7 subtypes and 1 respectively, which were 1 subtype A1, 35 subtype B, 19 subtype B, 3 subtype C, 49 CRF01_AE subtypes, 51 CRF07_BC subtypes and 3 CRF08_BC subtypes. There are already seven HIV-1 subtypes and epidemiologically recombinant forms in Beijing, and the surveillance of HIV-1 subtype epidemic in Beijing should be strengthened.