二硫化碳对雌性小鼠受精卵发育及染色体的影响

• 作 者: 保毓书 蒋衡 郑青 于永强 高敏 邵华
• 机 构: 北京医科大学公共卫生学院,北京医科大学公共卫生学院,北京医科大学公共卫生学院,化学工业部劳动保护研究所,化学工业部劳动保护研究所,化学工业部劳动保护研究所
• 出 处: 卫生毒理学杂志
• 发表日期: 1990年03期
• 关键词: 雌性小鼠 受精卵发育 染色体畸变 小鼠腹腔注射 雌原核 仔鼠 染色体分离 小鼠受精卵 雌性动物 发育异常

用昆明种性成熟雌性小鼠,以10mg/m~3及100mg/m~3浓度CS_2吸入染毒,每日2小时,每周6天,染毒3周(相当3～4个动情周期)后给雌小鼠腹腔注射孕马血清及HCG,令超数排卵,并与未经染毒雄性小鼠交配。后处死小鼠,检查受精卵并制备染色体标本。结果,两个染毒组,发育异常卵出现率显著高于对照组,雌原核染色体畸变的受精卵出现率也显著高于对照组。

Kunming sexual maturation of female mice, 10 mg / m ~ 3 and 100mg / m3 concentration of CS2 inhalation exposure, 2 hours a day, 6 days a week, exposure to 3 weeks (equivalent to 3 to 4 estrous cycles) After intraperitoneal injection of pregnant males serum and HCG to the female mice, so that the number of ovulation, and mate without injection of male mice. Mice were sacrificed, the fertilized eggs were examined and chromosomal specimens were prepared. The results showed that the incidence of abnormal eggs in the two exposure groups was significantly higher than that in the control group. The incidence of zygotes in the progenitor chromosomes was also significantly higher than that in the control group.