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目的:观察荞麦黄酮对1型糖尿病小鼠肾损伤的保护作用及其机制。方法:小鼠腹腔注射四氧嘧啶100mg/kg,每隔7d注射一次,连续3次,建立小鼠1型糖尿病肾病模型。将成模小鼠随机分为模型对照组、荞麦黄酮低、中、高剂量组(50、100、200mg/kg)及厄贝沙坦组(25mg/kg),另设正常组。造模后各组小鼠灌胃给药1次/d,连续4w。给药前及给药期间每隔1w用血糖仪测定小鼠空腹血糖(FBG),末次给药后肾称重,计算肾指数,采用全自动生化仪测定血清中血肌酐、尿素氮,采用ELISA测定尿微量白蛋白含量。肾组织匀浆测定糖基化终产物(AGEs),超氧化物歧化酶(SOD)及丙二醛(MDA)含量。HE染色观察各组小鼠肾组织形态。结果:与正常组比较,模型对照组小鼠空腹血糖连续4 w持续升高、血肌酐、血尿素氮、尿微量白蛋白、肾指数、肾组织中AGEs、MDA均明显增高,而SOD水平下降,且肾组织形态明显损伤。与模型对照组比较,荞麦黄酮在50~200mg/kg剂量范围均可改善上述指标,在100mg/kg剂量下作用更为明显。结论:荞麦黄酮对1型糖尿病小鼠肾损伤具有一定保护作用,其机制与降低体内AGEs、MDA水平,增加SOD水平相关。
Objective: To observe the protective effect of buckwheat flavonoids on renal injury in type 1 diabetic mice and its mechanism. Methods: The mice were injected intraperitoneally with alloxan 100 mg / kg and injected once every 7 days for 3 times to establish a mouse model of diabetic nephropathy. Mice were randomly divided into model control group, buckwheat flavonoids low, middle and high dose group (50,100,200mg / kg) and irbesartan group (25mg / kg), another set of normal group. After modeling, mice in each group were given gavage once daily for 4 weeks. The fasting blood glucose (FBG) in mice was measured every 1w before and during the administration. The kidneys were weighed after the last administration, and the renal index was calculated. Serum creatinine and urea nitrogen were measured by automatic biochemical analyzer. Determination of urinary albumin content. The contents of advanced glycosylation end products (AGEs), superoxide dismutase (SOD) and malondialdehyde (MDA) in renal tissue homogenate were measured. The morphology of kidney in each group was observed by HE staining. Results: Compared with the normal group, the fasting blood glucose of model control group continued to increase for 4 w continuously, serum creatinine, blood urea nitrogen, urine microalbumin, renal index, AGEs and MDA in renal tissue were significantly increased, while the SOD level was decreased , And renal tissue morphology was significantly damaged. Compared with the model control group, buckwheat flavonoids in the 50 ~ 200mg / kg dose range can improve the above indicators, the role of 100mg / kg dose is more obvious. CONCLUSION: Buckwheat flavonoids have a protective effect on kidney injury in type 1 diabetic mice. The mechanism is related to the reduction of AGEs and MDA in vivo and the increase of SOD level.