论文部分内容阅读
以自制月桂酸甘油酯为原料,选择超声乳化法制备了抗坏血酸类脂质体。以类脂质体包封率为指标,通过单因素实验考察了超声乳化法中胆固醇用量、药物用量、超声时间、稀释介质的温度对抗坏血酸类脂质体包封率的影响。通过单因素实验考察了超声时间、水相温度、胆固醇的用量、药物的用量对包封率的影响。结果表明,超声时间为15min,水相温度为0℃,脂质∶药物(w/w)为10∶1,类脂∶胆固醇(w/w)为2∶1为最优条件。实验表明,以自制的甘油二酯为原料能够形成包封率较好的类脂质体纳米药物载体,为类脂质体的合成提供了一种价格低廉的原料。
Using homemade laurin as raw material, ascorbic acid liposomes were prepared by ultrasonic emulsification. Based on the encapsulation efficiency of liposomes, the effects of cholesterol, drug dosage, ultrasonication time and dilution medium temperature on the encapsulation efficiency of ascorbic acid liposomes were investigated by single factor experiments. The single factor experiments were used to investigate the influence of ultrasonic time, water temperature, the amount of cholesterol and the dosage of drug on the entrapment efficiency. The results showed that the optimum conditions were as follows: ultrasonic time was 15 min, temperature of aqueous phase was 0 ℃, lipid: drug (w / w) was 10:1, lipid: cholesterol was 2:1. Experiments show that self-made diglyceride as raw material can form a good encapsulation rate of liposome-like nano-drug carrier for liposome-like synthesis provides a cheap raw materials.