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目的研究2,4,6-三硝基苯磺酸(TNBS)致肠炎大鼠的背根神经节(DRG)神经元电生理特性,为更全面地了解炎症性肠病(IBD)提供借鉴。方法 SD大鼠(雄性,体质量160~200 g)随机分为实验组和对照组,实验组(n=5)给予30%TNBS溶液(剂量40 mg/kg)灌肠,对照组(n=5)给予等效体积的生理盐水灌肠。在灌肠的第8天(炎症急性期)处死大鼠,对其结肠进行H-E染色,以确定造模是否成功;取其DRG神经元用全细胞膜片钳技术分析其电生理特性。结果实验组大鼠体质量降低(P<0.001),H-E染色示肠黏膜腺体结构严重破坏,炎性细胞浸润明显,表明造模成功。TNBS致肠炎后大鼠DRG神经元动作电位的阈电流降低(P<0.05)。结论 TNBS致肠炎后大鼠DRG神经元兴奋性增高。
Objective To study the electrophysiological characteristics of dorsal root ganglion (DRG) neurons in rats with 2,4,6-trinitrobenzene sulfonic acid (TNBS) -induced enteritis and provide references for more comprehensive understanding of inflammatory bowel disease (IBD). Methods SD rats (male, body weight 160-200 g) were randomly divided into experimental group and control group. The experimental group (n = 5) was given 30% TNBS solution (dose 40 mg / kg) ) Was given an equivalent volume of saline enema. On the 8th day of enema (acute phase of inflammation), rats were sacrificed and their colon was stained with H-E to determine if the model was successful. The DRG neurons were harvested and analyzed for their electrophysiological properties using whole-cell patch-clamp technique. Results The body weight of rats in the experimental group was decreased (P <0.001). The H-E staining showed that the glandular structure of the intestinal mucosa was severely damaged and the infiltration of inflammatory cells was obvious. The threshold current of DRG neuron action potential was decreased after TNBS injection (P <0.05). Conclusion The excitability of DRG neurons in rats after TNBS is increased.