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Aim:To prepare new pharmaceutical forms with sustained delivery properties ofrecombinant human bone morphogenetic protein-2(rhBMP_2)for tissue engineer-ing and guided tissue regeneration(GTR)use.Methods:rhBMP2-1oaded dextran-based hydrogel microspheres(rhBMP_2-MPs),which aimed to keep rhBMP2 bioac-tivity and to achieve long-term sustained release of rhBMP_2,were prepared bydouble-phase emulsified condensation polymerization.The physical,chemicalperformances and biological characteristics of those microspheres were studiedboth in vitro and in vivo.Results:The microspheres’ average diameter was30.33±4.32μm with 75.4% ranging from 20 μm to 40 μm and the drug loading andencapsulation efficiency were 7.82% and 82.25%,respectively.The rhBMP_2-releasing profiles in vitro showed that rhBMP_2 release could be maintained morethan 10 d.The rhBMP_2-MPs,with good swelling and biodegradation behavior,could be kept for 6 months at below 4 ℃ without significant characteristic changeor bioactivity loss.Cytology studies showed that rhBMP_2-MPs could promotethe proliferation of periodontal ligament cells(PDLCs)approximately 10 d,whilethe bioactivity of concentrated rhBMP_2 solution could keep no more than 3 d.Scanning electron microscope showed that rhBMP_2-MPs could be enchased intothe porous structure of calcium phosphate ceremic(CPC)and the eugonic growthof PDLCs in CPC/rhBMP_2-MPs scaffolds.Animal experiments indicated that us-ing CPC/rhBMP_2-MPs scaffolds could gain more periodontal tissue regenerationthan using rhBMP_2 compound firsthand with CPC(CPC/rhBMP_2).Conclusion:By encapsulating rhBMP_2 into dextran-based microspheres,a small quantity ofrhBMP_2 could achieve equivalent effects to the concentrated rhBMP_2 solutionand at the same time,could prolong rhBMP_2 retention both in vitro and in vivo.
Aim: To prepare new pharmaceutical forms with sustained delivery properties of recombinant human bone morphogenetic protein-2 (rhBMP_2) for tissue engineering and guided tissue regeneration (GTR) use. Methods: rhBMP2-1oaded dextran-based hydrogel microspheres (rhBMP_2- MPs) , which aimed to keep rhBMP2 bioac-tivity and to achieve long-term sustained release of rhBMP_2, were prepared by double-phase emulsified condensation polymerization. The physical, chemical performance and biological characteristics of those microspheres were studied in vitro and in vivo. Results: The microspheres’ average diameter was 30.33 ± 4.32 μm with 75.4% ranging from 20 μm to 40 μm and the drug loading andencapsulation efficiency were 7.82% and 82.25%, respectively.The rhBMP_2-releasing profiles in vitro showed that rhBMP_2 release could be more morehan 10 d.The rhBMP_2-MPs, with good swelling and biodegradation behavior, could be kept for 6 months at below 4 ℃ without significant characteristic changeor bioactivit While the bioactivity of concentrated rhBMP_2 solution could keep no more than 3 d. Scanning electron microscope showed that rhBMP_2-MPs could be enchased intothe porous structure of calcium phosphate ceremic (CPC) and the eugonic growth of PDLCs in CPC / rhBMP_2-MPs scaffolds.Animal experiments indicated that us-ing CPC / rhBMP_2-MPs scaffolds could gain more periodontal tissue regeneration using rhBMP_2 compound firsthand with CPC (CPC / rhBMP_2) .Conclusion: By encapsulating rhBMP_2 into dextran-based microspheres, a small quantity of rhBMP_2 could achieve equivalent effects to the concentrated rhBMP_2 solutionand at the same time, could prolong rhBMP_2 retention both in vitro and in vivo.