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PGE、F经过肺脏迅速地被细胞内胞浆酶所代谢,该酶主要是15-羟前列腺素脱氢酶。各种属的肺,正常时可把肺灌流的PGE_2清除,不能使PGE_2达到动脉做为一个循环激素的浓度。然而,未成熟的胎羊在循环中有高浓度的PGE_2。羊足月妊娠为150天,采用剖腹生产的8个妊娠95～107天未成熟的胎羊和10个妊娠136～147天近于成熟的胎羊,迅速放血杀死。做胎羊的左肺动脉和气管插管,迅速取出左肺并称重,悬挂在一定温度(38℃)、湿度的设备中。用改良的Krebs-Tris液以恒速非再循环系统从动脉灌注左肺,平衡以后,把~(14)C-菊粉和~3HPGE_2在15秒期间灌注进入肺脏。在流出的样品中,以15秒间期为一个样品,
PGE, F is rapidly metabolized by intracellular cytosolic enzymes, mainly 15-hydroxyproline dehydrogenase, through the lungs. Various genus of lung, normal can be perfusion of lung PGE2 clear, can not make PGE2 reach the artery as a circulating hormone concentration. However, immature fetuses have high concentrations of PGE 2 in the circulation. Sheep full-term pregnancy for 150 days, the use of caesarean section to produce 8 pregnant 95 to 107 days immature sheep and 10 pregnant 136 to 147 days near mature sheep, rapid blood to kill. Fetal sheep left pulmonary artery and endotracheal intubation, quickly remove the left lung and weighed, suspended in a certain temperature (38 ℃), humidity equipment. The left lung was perfused from the artery with a modified Krebs-Tris solution at a constant speed non-recirculation system and after the equilibration, ~ (14) C-inulin and ~ 3HPGE_2 were perfused into the lungs during 15 seconds. In the outflow of the sample to a 15 second period as a sample,