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单细胞光镊拉曼光谱技术测量单个红细胞的血红蛋白浓度,配制不同浓度的血红蛋白溶液后利用拉曼光谱强度与血红蛋白浓度的关系,测量单个红细胞的拉曼光谱,根据公式计算单个红细胞内血红蛋白浓度。测量了70个血红细胞的拉曼光谱,结果显示单个红细胞的血红蛋白浓度为270~500 g/L,其中血红蛋白浓度在350~450 g/L之间的是成熟的红细胞,占红细胞总数的90%以上,而浓度在300 g/L以下的可能是网织红细胞,浓度在450 g/L以上的是老化了的红细胞。这与平均红细胞血红蛋白浓度(MCHC)310~370 g/L基本一致。表明利用该方法可以对单个红细胞内的血红蛋白浓度进行快速、无损、简单、精确的定量分析,展现了拉曼光谱技术在单细胞分析上的优势。
Single-cell optical tweezers Raman spectroscopy measurement of individual hemoglobin concentration of red blood cells, the preparation of different concentrations of hemoglobin solution using the relationship between the intensity of Raman spectroscopy and hemoglobin concentration, single Raman spectroscopy measurement of individual erythrocytes according to the formula to calculate the hemoglobin concentration. The Raman spectra of 70 red blood cells were measured. The results showed that the single hemoglobin concentration of erythrocytes was 270-500 g / L, of which the hemoglobin concentration was between 350-450 g / L. The mature erythrocytes accounted for 90% of the total number of red blood cells, , While the concentration below 300 g / L may be reticulocytes, the concentration of 450 g / L and above is the aging of red blood cells. This is consistent with the average erythrocyte hemoglobin concentration (MCHC) 310 ~ 370 g / L. This method shows that this method can rapidly, non-destructive, simple and accurate quantitative analysis of hemoglobin concentration in a single erythrocyte, which shows the advantages of Raman spectroscopy in single-cell analysis.