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目的:改良建立小鼠哮喘模型的方法并评价其效果。方法:清洁级BALB/c小鼠20只,随机分为模型组和对照组,模型组于第0和14d注射OVA(卵清蛋白)致敏,第21~25天雾化吸入5%OVA激发,对照组用生理盐水替代。结果:模型组在激发过程中出现明显头面部瘙痒,呼吸加深加快,安静少动,弓背,前肢缩抬等哮喘急性发作表现。BALF(支气管肺泡灌洗液)中白细胞总数及嗜酸性粒细胞分类明显增多。血清及BALF中IL-4明显增高,INF-γ下降,OVA特异性IgE增高。肺组织病理切片见小支气管及伴行血管周围有较多炎症细胞浸润,可见大量嗜酸性粒细胞,杯状细胞增生,平滑肌增厚,部分肺泡间隔融合形成肺气肿。结论:通过对小鼠哮喘模型建立方法的改良,成功复制出小鼠哮喘发作的动物模型。
Objective: To improve the method of establishing mouse asthma model and evaluate its effect. Methods: Twenty BALB / c clean mice were randomly divided into model group and control group. The model group was sensitized with OVA (ovalbumin) on day 0 and 14 and challenged with 5% OVA inhalation on days 21-25 , The control group with saline instead. Results: The acute exacerbation of asthma in the model group showed obvious pruritus, deepening respiration, quiet and less motility, dorsal bowel and forelimb contraction. BALF (bronchoalveolar lavage fluid) in the total number of leukocytes and eosinophils increased significantly. Serum and BALF IL-4 was significantly increased, INF-γ decreased, OVA-specific IgE increased. Pathological sections of lung tissue seen in the bronchus and associated vessels around the more inflammatory cell infiltration, showing a large number of eosinophils, goblet cell hyperplasia, smooth muscle thickening, part of the alveolar septum fusion emphysema. Conclusion: The animal model of mouse asthma attack was successfully replicated through the improvement of the establishment of mouse asthma model.