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目的探讨本地区汉族人肝脂酶(HL)基因启动子250G/A多态性与冠心病的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RELP)技术检测了103例冠心病患者和69名健康对照组汉族人HL启动子250G/A多态性基因型,探讨了其对血脂、载脂蛋白水平的影响。结果冠心病组的基因型和等位基因频率分布与正常老年组之间的差异无统计学意义;冠心病组的高密度脂蛋白胆固醇(HDL-C)载脂蛋白A1(apoA1)水平与正常老年组同基因型之间的差异具有统计学意义(P<0.05);冠心病组的apoA1水平(1.20±0.31)g/L明显低于正常老年组的同基因型(1.49±0.23)g/L;冠心病组的HDL-C水平(1.30±0.31)mmol/L明显高于同组的无突变型(1.16±0.32)mmol/L。经多元Logistic逐步回归分析的显示,TG是冠心病危险因子,apoA1是保护因子(P<0.05),但未见A等位基因是一个危险因子。结论在冠心病患者中,HL启动子250G/A基因多态性对血脂水平有一定影响。
Objective To investigate the association between the 250G / A polymorphism of the hepatic lipase (HL) gene promoter and coronary heart disease in Han nationality in the region. Methods PCR-RELP method was used to detect the polymorphism of 250G / A polymorphism of HLG promoter in 103 patients with coronary heart disease and 69 healthy controls. Blood lipids, apolipoprotein levels. Results There was no significant difference in the distribution of genotype and allele between CHD patients and normal controls. The level of HDL-C apolipoprotein A1 (apoA1) There was significant difference between genotypes in the elderly group (P <0.05). The level of apoA1 in coronary heart disease group (1.20 ± 0.31) g / L was significantly lower than that in the normal group (1.49 ± 0.23) g / L; HDL-C level in coronary heart disease group (1.30 ± 0.31) mmol / L was significantly higher than that in the same group (1.16 ± 0.32) mmol / L. Logistic stepwise regression analysis showed that TG was a risk factor for coronary heart disease and apoA1 was a protective factor (P <0.05), but no A allele was a risk factor. Conclusion In patients with coronary heart disease, the 250G / A gene polymorphism of HL promoter may have some influence on the blood lipid level.