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目的建立小鼠结肠癌肝转移模型,并研究肝星状细胞(hepatic stellete cell,HSC)与结肠癌肝转移的相关性。方法采用囊袋法瘤块盲肠原位移植建立15只小鼠结肠癌肝转移模型,免疫组化法观察活化的HSC在转移灶、癌旁组织及未转移肝组织内的表达情况。结果建模后3周结肠癌肝转移率为40%,4周转移率为60%。转移灶中活化HSC的表达显著高于癌旁组织和未转移肝组织。结论囊袋法瘤块盲肠原位移植是较理想的结肠癌肝转移模型的制作方法;肝脏局部免疫微环境中活化HSC的表达可能参与结肠癌肝转移过程中的免疫调控。
Objective To establish a model of hepatic metastasis in mice with colon cancer and study the correlation between hepatic stellate cell (HSC) and hepatic metastasis of colon cancer. Methods 15 mice with colon cancer liver metastasis were established by orthotopic transplantation of bladder tumor by capsular bag method. The expression of activated HSC in metastatic lesions, paracancer tissues and non-metastatic liver tissues was observed by immunohistochemistry. Results Three weeks after modeling, the liver metastasis rate of colon cancer was 40%, and the metastasis rate of 4 weeks was 60%. The expression of activated HSC in metastatic lesions was significantly higher than that in paracancerous tissues and non-metastasized liver tissues. Conclusions Occult implantation of capsulotomy tumor is an ideal method to produce liver metastasis of colon cancer. The expression of activated HSC in the local immune microenvironment may be involved in the immune regulation of liver metastasis of colon cancer.