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目的 探讨云南汉族系统性红斑狼疮 (systemic lupus erythematosus,SL E)患者自身抗体与HL A- 抗原的相关性。方法 用单克隆抗体微量淋巴毒试验对 5 7例云南汉族 SL E患者进行 HL A- 类DR、DQ抗原特异性血清学分型 ,用免疫金渗滤试验检测抗 ds- DNA抗体 ,免疫印迹法检测可提取核抗原抗体。结果 抗 ds- DNA抗体阳性者的 DR9、DQ2抗原频率分别为 45 .71% (16 /35 )、40 .0 0 % (14/35 ) ,显著高于抗 ds- DNA阴性者。抗 SSA和抗 SSB阳性者的 DR14、DQ5抗原频率分别为 2 8.5 7% (4 /14)、35 .76 %(5 /14) ,显著高于抗 SSA和抗 SSB阴性者。抗 U1核糖核蛋白 (U1ribonuleoprotein,U1RNP)阳性者的DQ6抗原频率为 12 .5 0 % (2 /16 ) ,显著低于抗 U1RNP阴性者 ;DQ7抗原频率为 75 .0 0 % (12 /16 ) ,显著高于抗 U1RNP阴性者。结论 云南汉族 SL E患者自身抗体与 HL A- DR、DQ抗原的关联性有自己的地区特异性。
Objective To investigate the correlation between autoantibodies and HL-A antigens in patients with systemic lupus erythematosus (SL E) in Yunnan Han nationality. Methods HLA-DR and DQ antigen specific serotypes were detected in 47 RA patients with Yunnan Han SLR by monoclonal antibody lymphotoxin test. Anti-dsDNA antibody was detected by immunogold filtration assay and detected by immunoblotting Can extract nuclear antigen antibody. Results The frequencies of DR9 and DQ2 antigens in anti-dsDNA antibody were 45.71% (16/35) and 40.0% (14/35) respectively, which were significantly higher than those in anti-dsDNA negative. The frequencies of DR14 and DQ5 antigens in SSA and SSB positive patients were 2 8.57% (4/14) and 35.76% (5/14), respectively, which were significantly higher than those in anti-SSA and anti-SSB. The frequency of DQ6 antigen in U1RNP positive patients was 12.5% (2/16), which was significantly lower than that in U1RNP negative patients. The frequency of DQ7 antigen was 75.0% (12/16) , Significantly higher than anti-U1RNP negative. Conclusions The association of autoantibodies to HL-DR and DQ antigens in SL E patients of Yunnan Han has its own regional specificity.