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目的探讨海参虫草复合物(Apostichopus japonicus and Cardyceps millitaris,AC)对糖尿病大鼠的降血糖作用。方法通过一次性腹腔注射链脲佐菌素(streptozocin,STZ)建立糖尿病大鼠模型,将成模大鼠随机分为模型对照组、海参虫草复合物低剂量组、高剂量组。灌胃海参虫草复合物35 d后,分别检测大鼠空腹血糖值和血清胰岛素含量;采用RT-PCR法测定肌肉及脂肪组织中磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)、蛋白激酶(serine-threonine kinases,Akt)和葡萄糖转运蛋白4(glucose transporter 4,Glut4)mRNA的表达,Western blot方法检测肌肉及脂肪中Glut4蛋白的表达。结果海参虫草复合物能显著降低糖尿病大鼠空腹血糖(P<0.01),增加血清胰岛素含量(P<0.01),上调肌肉及脂肪组织中PI3K、Akt和Glut4的mRNA表达(P<0.05或P<0.01),增高脂肪组织中Glut4的蛋白表达水平(P<0.01)。结论海参虫草复合物具有降低血糖的作用,其机制可能与部分上调胰岛素介导的PI3K/Akt/Glut4信号转导通路有关。
Objective To investigate the hypoglycemic effect of Apostichopus japonicus and Cardyceps millitaris (AC) on diabetic rats. Methods A diabetic rat model was established by intraperitoneal injection of streptozocin (STZ). The model rats were randomly divided into a model control group, a low-dose group and a high-dose group. The gavage of Cordyceps militaris compound was administered for 35 days, and the fasting blood glucose and serum insulin were detected respectively. The levels of phosphatidylinositol 3-kinase (PI3K) and protein Serine-threonine kinases (Akt) and glucose transporter 4 (Glut4) mRNA were detected by Western blot. Glut4 protein expression in muscle and adipose tissue was detected by Western blot. Results The sea cucumber cordyceps compound could significantly decrease fasting blood glucose (P <0.01), increase serum insulin (P <0.01) and up-regulate the mRNA expressions of PI3K, Akt and Glut4 in muscle and adipose tissue (P <0.05 or P < 0.01), increased the expression of Glut4 in adipose tissue (P <0.01). Conclusion The cucumber cordyceps compound has a hypoglycemic effect, and its mechanism may be related to the up-regulation of insulin-mediated PI3K / Akt / Glut4 signal transduction pathway.