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目的探讨黏病毒抗性蛋白1(MxA)基因多态性与人群严重急性呼吸综合征(SARS)发病间的关系。方法采用病例对照研究,用聚合酶链反应限制性片段长度多态性分析检测mxA基因启动子88位G/T多态性位点,对SARS传播的相关因素进行问卷调查,并用SPSS软件进行单因素和多因素分析。结果选取了66例SARS病例和64名对照进行研究。与GG基因型相比,含等位基因T的基因型(GT+TT)在病例中的构成比(81.3%)显著高于对照组(62.5%),OR值(95%CI)为2.700(1.208～6.037)。多因素logistic回归分析结果表明,在调整了与SARS病例接触时戴口罩,穿防护服,戴眼罩等相关因素后,与GG基因型相比,含等位基因T的基因型(GT+TT)仍与SARS发病有显著性关联,调整OR值(95%CI)为2.911(1.027～8.250)。结论mxA基因启动子88位G/T多态性可能与中国汉族人群SARS发病的基因易感性有关。
Objective To investigate the relationship between the MxA polymorphism and the incidence of severe acute respiratory syndrome (SARS). Methods A case-control study was conducted to detect the polymorphic site of 88 allele of mxA gene by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. The related factors of SARS transmission were investigated by questionnaire and SPSS software was used for single Factors and multivariate analysis. Results 66 SARS cases and 64 controls were selected for the study. The odds ratio (GT + TT) of genotypes with allele T (81.3%) was significantly higher than that of controls (62.5%) and OR (95% CI) was 2.700 (95% CI) compared with GG genotype 1.208 ~ 6.037). Multivariate logistic regression analysis showed that compared with the GG genotype, the allele T-containing genotype (GT + TT) was significantly lower than that of the GG genotype The incidence of SARS was still significantly associated with adjustment OR (95% CI) was 2.911 (1.027 ~ 8.250). Conclusion The 88 G / T polymorphism of mxA gene promoter may be related to the susceptibility to SARS in Chinese Han population.