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目的：研究端粒酶活性在子宫颈癌组织发生、发展中的作用及端粒酶活性的检测在筛查子宫颈癌中的临床意义。方法：应用多聚酶链反应酶联免疫吸附法（ＰＣＲＥＬＩＳＡ）对２１份子宫颈癌组织，１０份正常子宫颈组织及不同浓度的ＨｅＬɑ细胞株的端粒酶活性进行检测。结果：在１０份正常子宫颈组织中均未测出端粒酶活性；２１份子宫颈癌组织中２０份有端粒酶活性阳性表达；在所有ＨｅＬɑ细胞数≥１００的样本中端粒酶活性均为阳性表达。结论：端粒酶活性在子宫颈癌发生和发展中起重要作用；端粒酶活性的检测也有助于子宫颈癌的筛查。
Objective: To study the role of telomerase activity in the pathogenesis and development of cervical cancer and the clinical significance of the detection of telomerase activity in the screening of cervical cancer. Methods: The telomerase activity of 21 cervical cancer tissues, 10 normal cervical tissues and HeLa cells of different concentrations was detected by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA). RESULTS: Telomerase activity was not detected in 10 normal cervical tissues; telomerase activity was positive in 20 of 21 cervical cancer tissues; telomerase activity was found in all HeL cells with ≥ 100 samples. For positive expression. Conclusion: Telomerase activity plays an important role in the occurrence and development of cervical cancer. The detection of telomerase activity also contributes to the screening of cervical cancer.