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目的研究一氧化氮(NO)对体外培养的人骨肉瘤细胞株(OS-732)侵袭能力的影响。方法将含有不同浓度硝普钠(SNP)的骨肉瘤细胞株悬液加入肿瘤体外侵袭模型中,分别计数细胞跨膜侵袭的数量,分析不同浓度SNP组间细胞跨膜侵袭数量的差异及与对照组间的差异。结果随着SNP浓度的增加,人骨肉瘤细胞株细胞穿过膜的数量呈逐渐减少的趋势,变化范围从平均每个视野184.4±12.8降至33.0±9.0,而PBS组平均为每个视野195.8±14.5。经统计分析,除SNP500μmol/L组与其他各组间均存在显著性差异(P<0.05)外,其余各组(包括PBS组)均与非相邻组间存在显著性差异(P<0.05),而与相邻组间无显著性差异(P>0.05)。结论NO可以在一定程度上抑制骨肉瘤细胞的体外跨膜侵袭能力。
Objective To investigate the effect of nitric oxide (NO) on the invasiveness of human osteosarcoma cell line (OS-732) cultured in vitro. Methods Osteosarcoma cell suspension containing different concentration of sodium nitroprusside (SNP) was added into the in vitro invasion model of tumor. The number of transmembrane invasion was counted, and the difference of cell transmembrane invasion between SNPs of different concentration and the control Differences between groups. Results With the increase of SNP concentration, the number of cells in human osteosarcoma cell line decreased gradually from 184.4 ± 12.8 to 33.0 ± 9.0 in each field of vision, while the average in PBS group was 195.8 ± 14.5. Statistical analysis showed there was a significant difference (P <0.05) between other groups (including PBS group) and non-adjacent groups except SNP500μmol / L group and other groups (P <0.05) , But no significant difference with adjacent groups (P> 0.05). Conclusion NO can inhibit the transmembrane invasion ability of osteosarcoma cells to a certain extent.