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以籼稻为材料,研究了γ-射线对水稻不同外植体(成熟胚、幼胚、幼穗等)愈伤组织诱导率、分化率和结实率的影响,结果表明:幼胚和幼穗在培养之前,进行γ-射线处理,照射量不宜超过0.258C/kg;用γ-射线7.740C/kg照射后的M_1植株幼穗作外植体,可提高培养力;成熟胚在培养前进行辐射处理,会降低出愈率,但分化率却明显提高,其照射量以0.645～1.290C/kg为宜,如果直接辐照愈伤组织,则以6.45×10~(-2)C/kg为佳.辐射处理会促进意伤组织老化,缩短分化历期,一般幼穗愈伤组织的有效分化期为接种后150～180天.
The effects of γ-rays on the callus induction rate, differentiation rate and seed setting rate of different explants (mature embryo, immature embryo, young ear, etc.) of rice were studied using indica rice. The results showed that: Before culturing, γ-ray treatment should be carried out with the irradiation dose not exceeding 0.258C / kg; the young ear of M_1 plant irradiated with γ-ray of 7.740C / kg can improve the culture ability; the mature embryo is irradiated before culturing Treatment, will reduce the rate of the outbreak, but the differentiation rate was significantly improved, the irradiation dose of 0.645 ~ 1.290C / kg is appropriate, if the direct irradiation of callus, the 6.45 × 10 -2 C / kg Radiation treatment will promote the aging of the injured tissue and shorten the differentiation period, and the effective differentiation period of young panicle callus generally ranges from 150 to 180 days after inoculation.