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目的研究谷胱苷肽S转硫酶T1、M1和P1(GSTT1、GSTM1和GSTP1)多态性与结直肠癌易感性的关系。方法在江苏省进行了1个病例—对照研究(结直肠癌患者315例,人群对照439例),调查研究对象的生活习惯,抽取静脉血,提取白细胞DNA,以多重PCR技术检测GSTT1和GSTM1基因缺失,PCR-RFLP技术检测GSTP1基因单核苷酸多态(第104密码子A→G)。结果①在结直肠癌组和正常组GSTT1和GSTM1基因缺失频率分别为55.24%、57.31%和72.70%、73.29%、差异无显著性。②在结直肠癌组GSTP1 A/A、A/G和G/G基因型分布频度分别为57.51%、36.74%和5.75%;对照组分别为63.70%、31.05%和5.25%,2组之间差异无显著性(χ2 MH=2.993,P=0.224)。与GSTP1 A/A基因型携带者相比,G/G基因型者发生结直肠癌的危险性无显著升高,其性别、年龄、吸烟、饮酒习惯调整后的OR为1.09(95%CI:0.79~1.51)。结论GSTT1、GSTM1基因缺失型和GSTP1 G/G基因型与结直肠癌的易感性无显著相关。
Objective To investigate the relationship between polymorphisms of glutathione S-transferases T1, M1, and P1 (GSTT1, GSTM1, and GSTP1) and susceptibility to colorectal cancer. Methods A case-control study (315 patients with colorectal cancer and 439 control subjects) was performed in Jiangsu Province. The living habits of the study subjects were investigated. Venous blood was collected and leukocyte DNA was extracted. GSTT1 and GSTM1 genes were detected by multiplex PCR. Deletion, PCR-RFLP detection of GSTP1 gene single nucleotide polymorphism (codon 104 codon A → G). Results 1 The frequencies of GSTT1 and GSTM1 deletion in colorectal cancer and normal group were 55.24%, 57.31%, 72.70% and 73.29%, respectively. There was no significant difference between the two groups. 2The frequencies of GSTP1 A/A, A/G, and G/G genotypes in colorectal cancer were 57.51%, 36.74%, and 5.75%, respectively; in the control group, they were 63.70%, 31.05%, and 5.25%, respectively. There was no significant difference between the two groups (χ2 MH=2.993, P=0.224). Compared with the GSTP1 A/A genotype carrier, the G/G genotype had no significant increase in the risk of colorectal cancer, and its adjusted OR for sex, age, smoking, and drinking habits was 1.09 (95% CI: 0.79~1.51). Conclusion There is no significant correlation between GSTT1, GSTM1 gene deletion and GSTP1 G/G genotypes and susceptibility to colorectal cancer.