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AIM To investigate the expression and significance of P-FEN,hypoxia-inducible factor-1 alpha(HIF-1α),and targetinggene VEGF during colorectal carciogenesis.METHODS:Total 71 cases colorectal neoplasms(9 casesof colorectal adenoma and 62 colorectal adenocarcinoma)were formalin fixed and paraffin-embedded,and allspecimens were evaluated for PTEN mRNA,HIF-1α mRNAand VEGF protein expression.PTEN mRNA,HIF-1α mRNAwere detected by in situ hybridization.VEGF protein wasidentified by citrate-microwave SP immunohistochemicalmethod.RESULTS:There were significant differences in PTEN,HIF-1α and VEGF expression between colorectal adenomas andcolorectal adenocarcinoma(P<0.05).The level of PTENexpression decreased as the pathologic stage increased.Conversely,HIF-1α and VEGF expression increased withthe Dukes stage as follows:stage A(0.1029±0.0457:0.1207±0.0436),stage B(0.1656±0.0329:0.1572±0.0514),and stage C+D(0.2335±0.0748:0.2219±0.0803).For PTENexpression,there was a significant difference among Dukesstage A,B,and C+D,and the level of PTEN expressionwas found to be significant higher in Dukes stage A or Bthan that of Dukes stage C or D.For HIF-1α expression,there was a significant difference between Dukes stage Aand B,and the level of HIF-1α expression was found to besignificantly higher in Dukes stage C+D than that of Dukesstage A or B.The VEGF expression had similar results asHIF-1α expression.In colorectal adenocarcinoma,decreased levels of PTEN were significantly associated withincreased expression of HIF-1α mRNA(r=-0.36,P<0.05)and VEGF protein(r=-0.48,P<0.05)respectively.The levelsof HIF-1 were positively correlated with VEGF expression(r=0.71,P<0.01)CONCLUSION: Loss of PTEN expression and increased levels of HIF-la and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.
AIM To investigate the expression and significance of P-FEN, hypoxia-inducible factor-1 alpha (HIF-1α), and targetinggene VEGF during colorectal carciogenesis. METHODS: Total 71 cases colorectal neoplasms (9 casesof colorectal adenoma and 62 colorectal adenocarcinoma) were formalin fixed and paraffin-embedded, and allspecimens were evaluated for PTEN mRNA, HIF-1α mRNA and VEGF protein expression. PTEN mRNA, HIF-1α mRNA were detected by in situ hybridization. VEGF protein wasidentified by citrate-microwave SP immunohistochemical method. Significant differences in PTEN, HIF-1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma (P <0.05). The level of PTEN expression decreased as the pathologic stage increased. (0.1029 ± 0.0457: 0.1207 ± 0.0436), stage B (0.1656 ± 0.0329: 0.1572 ± 0.0514), and stage C + D (0.2335 ± 0.0748: 0.2219 ± 0.0803). For PTENexpression, there was a significant differenc e among Dukesstage A, B, and C + D, and the level of PTEN expression was found to be significantly higher in Dukes stage A or Bthan that of Dukes stage C or D. For HIF-1α expression, there was a significant difference between Dukes stage A and B, and the level of HIF-1α expression was found to be besificantly higher in Dukes stage C + D than that of Dukesstage A or B. The VEGF expression had similar results as HIF-1α expression. In colorectal adenocarcinoma, decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA (r = -0.36, P <0.05) and VEGF protein (r = -0.48, , P <0.01) CONCLUSION: Loss of PTEN expression and increased levels of HIF-la and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.