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AIM:To study and clone a novel liver cancer related gene,and to explore the molecular basis of liver cancer genesis.METHODS:Using mRNA differential display polymerssechain reaction(DDPCR),we investigated the difference ofmRNA in human hepstocallular carcinoma(HCC)and pairedsurrounding liver tissues,and got a gene probe.Byscreening a human placenta cDNA library and genornichomologous extend,we obtained a full-length cDNA namedHCCA3.We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues,anddistribution in human normal tissues by mssns of Northernblot assay.RESULTS:A full-length cDNA of liver cancer associated geneHCCA3 has been submitted to the GeneBank nucleotidesequence databases(Accession No.AF276707).Thepositive expression rate of this gene was 78.6%(33/42)inHCC tissues,and the clinical pathological data showed thatthe HCCA3 was closely associated with the invasion oftumor capsule(P=0.023)and adjacant small metastasissatellite nodules lesions(P=0.041).The HCCA3 was widelydistributed in the human normal tissues,which wasIntensively expressed In lungs,brain and colon tissues,while lowly expressed In the liver tissues.CONCLUSION:A novel full-length cDNA was cloned anddifferentiated,which was highly expressed in liver cancertissues.The high expression was closely related to thetumor invssivensss and metastasis,that may be the lateheredited change in HCC genssis.
AIM:To study and clone a novel liver cancer related gene,and to explore the molecular basis of liver cancer genesis.METHODS:Using mRNA differential display polymerssechain reaction(DDPCR),we investigate the difference of mRNA in human hepstocallular carcinoma(HCC) and pairedsurrounding Liver tissues,and got a gene probe.Byscreening a human placenta cDNA library and genornichomologous extend,we obtained a full-length cDNA namedHCCA3.We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues,anddistribution in human normal Tissues by mssns of Northernblot assay.RESULTS:A full-length cDNA of liver cancer associated geneHCCA3 has been submitted to the GeneBank nucleotides databases(Accession No.AF276707).Thepositiveexpressionrateofthisgene was 78.6%(33/42)inHCC tissues ,and the clinical pathological data showed that the HCCA3 was closely associated with the invasion of tumor capsule(P=0.023)and adjacant small metastasissatellite nodules lesion s(P=0.041).The HCCA3 was widelydistributed in the human normal tissues,which was Intensively expressed In lungs,brain and colon tissues,while lowly expressed In the liver tissues.CONCLUSION:A novel full-length cDNA was cloned anddifferentiated,which was Highly highly expressed in liver cancertissues. The high expression was closely related to the tumor in vsssivensss and metastasis, that may be the lateheredited change in HCC genssis.