论文部分内容阅读
随着全球的经济发展和人口膨胀,水资源短缺现象日趋严重,干旱己成为粮食生产的主要限制因素之一.C4植物(如玉米)具有较高的光合速率以及较高的水分利用效率,利用转基因技术将C4植物高光合及耐旱特征引入C3作物(如水稻),被认为是解决水资源短缺与全球粮食需求不断增长矛盾的重要手段.本研究以转PEPC+PPDK双基因水稻及其非转基因对照为材料,通过PEG6000模拟干旱条件,研究干旱胁迫下叶片叶绿素含量、叶绿素荧光参数、C4光合酶活性、叶片抗氧化酶活性等光合生理相关参数变化.结果表明:干旱胁迫下转PEPC+ PPDK基因水稻叶绿素含量、C4光合酶活性、抗氧化能力等均有提升;干旱胁迫对转PEPC+PPDK基因水稻PSⅡ的光化学活性抑制较小,对细胞的损伤相对更低,表明转基因水稻对光合机构的保护能力明显增强.“,”With the global economic development and expansion of the population,the shortage of water resources is becoming more and more serious,and the drought has become one of the main limiting factors of food production.Because the C4 plant (such as the corn) has higher photosynthetic rate and higher water use efficiency,it is considered to be an important method to solve the contradiction of the shortage of water resources and the growing demand of the global food,that the main characteristic of high photosynthetic and drought tolerance in the C4 plant is introduced into C3 crop (such as the rice) through using the transgenic technology.In this study,the author uses the comparison of the PEPC+PPDK transgenic rice and non-transgenic of it as materials,simulate drought conditions through the PEG6000,and research the changes of chlorophyll content,chlorophyll fluorescence parameters,C4 photosynthetic enzyme activities and antioxidant enzyme activities on the leaf.The results show that under the stress of the drought,the transgenic rice has been promoted in many respects,such as C4 photosynthetic enzyme activity,chlorophyll content and antioxidant capacity and so on,that profits from PEPC+ PPDK's bi-gene,and under the common action of these factors,it has reduced the inhibition of PSII's photochemical activity on account of drought's stress,and it has reduced the damage on the cell because of the drought's stress.The results indicate that the capability of genetically modified rice in protecting the photosynthetic apparatus has been dramatically enhanced.