培养表皮细胞膜片漂浮存放时基底细胞增殖受抑制

• 作 者: 戴方平
• 出 处: 国外医学.创伤与外科基本问题分册
• 发表日期: 1992年02期
• 关键词: 表皮细胞 基底细胞 BrdU 免疫标记 脱氧尿苷 掺入法 磷酸缓冲液 DNA 培养瓶 保存时间

培养的表皮细胞膜片用1.2U/ml Dispa-se Ⅱ在37℃的无血清培养液中孵育1小时。整片消化下来后,用磷酸缓冲液(PBS)洗两遍,移至37℃培养液中保存0～24小时,研究经不同时间保存后DNA的合成。5-溴-2脱氧尿苷(BrdU)掺入和免疫标记法是主要方法之一。取保存在培养液中漂浮0、2、4、6、8、12或24小时的细胞膜片,移至合10μm BrdU的培养液中37℃

The cultured epidermal cell membrane was incubated with 1.2 U / ml Dispa-se II for 1 hour in a serum-free medium at 37 ° C. After the whole piece is digested, the solution is washed twice with phosphate buffered saline (PBS) and stored in a 37 ° C culture solution for 0-24 hours to study the DNA synthesis after being stored at different times. Bromine-2-deoxyuridine (BrdU) incorporation and immunolabeling are among the major approaches. Cells stored in culture medium for 0, 2, 4, 6, 8, 12, or 24 hours were transferred to a culture broth of 10 μm BrdU at 37 ° C.