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拟南芥赤霉素2–氧化酶基因AtGA2ox1在矮牵牛(Petunia hybrida)中过量表达导致转基因植株开花延迟,株形明显矮化,花冠变小,花冠表皮细胞变小,但花色变化不明显。施用多效唑对矮牵牛花冠的影响与过量表达AtGA2ox1一致。用拟南芥茎特异性表达基因At3g56700的启动子驱动AtGA2ox1在矮牵牛中表达时,转基因植株的表型变化在株系间存在明显的差异,但外源基因在茎中的表达量都明显高于叶和花中的,果实和种子的发育未受影响,通过对转基因后代的筛选可以获得株形矮化,其它性状的发育受影响较小的转基因株系。
The overexpression of AtGA2ox1, a gene encoding GA3 in Petunia hybrida, resulted in delayed flowering, reduced plant shape, smaller corolla, smaller corolla epidermal cells but not obvious change of flower color . Effect of paclobutrazol on petunia corolla was consistent with overexpression of AtGA2ox1. When AtGA2ox1 was driven by At3g56700 promoter in Arabidopsis thaliana and expressed in petunia, the phenotypic changes of transgenic plants were significantly different among the lines, but the expression of exogenous genes in the stems was significant Higher than leaves and flowers, fruit and seed development was not affected, through the screening of transgenic offspring can be dwarfed, other traits less affected development of transgenic lines.