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目的了解STR基因座的多态性,评价其在法医鉴定中的应用价值。方法应用荧光标记试剂盒,对963例中国汉族无关个体的血样DNA进行D18S1364、D12S391、D13S325、D6S1043、D2S1772、D11S2368、D22-GATA198B05、D8S1132和D7S3048 9个基因座的复合扩增,用AB I 3100遗传分析仪对扩增产物进行电泳,GeneM apper 3.1分析软件指定等位基因型。并计算各个基因座的等位基因频率,统计其遗传学参数。结果 9个STR基因座共检出126个等位基因、536种基因型。各个基因座的等位基因数为11~20个,基因频率为0.001~0.289,杂合度为0.818~0.891,三联体非父排除率为0.633~0.777,二联体非父排除率为0.325~0.578,个人识别率为0.927~0.971。9个STR基因座的累积个人识别率为0.999999999999676,三联体累积非父排除率为0.99997665,二联体累积非父排除率为0.99752。经Hardy-W e inberg平衡检验,9个STR基因座均为P>0.05。结论该9个STR基因座在中国汉族人群中均属于多态性程度高的遗传标记,可以作为COD IS系统的补充。
Objective To understand the STR loci polymorphism and evaluate its value in forensic identification. Methods Ninety-three loci of D18S1364, D12S391, D13S325, D6S1043, D2S1772, D11S2368, D22-GATA198B05, D8S1132 and D7S3048 were amplified by fluorescent labeling kit from 963 Chinese Han unrelated individuals. Genetic Analyzer Electrophoresis of amplified product, GeneM apper 3.1 analysis software specified allele. And calculate the allele frequency of each locus, statistical genetic parameters. Results A total of 126 alleles and 536 genotypes were detected in the 9 STR loci. The number of alleles at each locus ranged from 11 to 20, with a frequency of 0.001 to 0.289, a heterozygosity of 0.818 to 0.891, a non-parent exclusion rate of 0.633 to 0.777 in triplets, and a non-parent exclusion rate of 0.325 to 0.578 , The individual recognition rate was 0.927 ~ 0.971. The cumulative personal recognition rate of 0.9 STR loci was 0.999999999999676, the triple non-father cumulative removal rate was 0.99997665, and the binary non-father cumulative elimination rate was 0.99752. After Hardy-W einberg equilibrium test, all 9 STR loci were P> 0.05. Conclusion These nine STR loci belong to the genetic markers with high degree of polymorphism in Chinese Han population and can be used as a complement to COD IS system.