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目的对比广西壮、汉民族HLA-DRB1*07基因与乙肝疫苗免疫应答的相关性。方法以广西73名壮族、97名汉族健康大学生作为研究对象进行全程乙肝疫苗接种,采用PCR-SSP技术检测受试者外周血细胞DNA的HLA-DRB1*07基因,采用ELISA方法检测其血清乙肝病毒标志物(HBsAg,抗-HBs,HBeAg,抗-HBe,抗-HBc)。结果壮族与汉族之间乙肝疫苗的应答率无显著性差别(84.21%vs 86.60%,P=0.658),壮族与汉族人群之间HLA-DRB1*07基因携带频率的差异也无统计学意义(9.21%vs 6.19%,P=0.454),但壮、汉族中HLA-DRB1*07携带者的疫苗应答率都显著低于非携带者(P=0.00 P/=0.03)。结论民族差异不是广西人群乙肝疫苗应答效果的影响因素;广西壮、汉族人中携带HLA-DRB1*07基因都可能是导致机体对乙肝疫苗无/弱应答的因素。
Objective To compare the correlation between HLA-DRB1 * 07 gene in Guangxi Zhuang and Han nationality and the immune response of hepatitis B vaccine. Methods A total of 73 Zhuang and 97 Han healthy college students in Guangxi were enrolled in this study. Hepatitis B vaccination was performed in all subjects. PCR-SSP was used to detect HLA-DRB1 * 07 gene in peripheral blood DNA of the subjects. ELISA was used to detect the serum hepatitis B virus (HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc). Results The response rate of HLA-DRB1 * 07 between Zhuang and Han nationality was not significantly different (84.21% vs 86.60%, P = 0.658) (9.21 % vs 6.19%, P = 0.454). However, the vaccine response rates of HLA-DRB1 * 07 carriers in both Zhuang and Han were significantly lower than those of non-carriers (P = 0.00 P = 0.03). Conclusion Ethnic difference is not the influencing factor of hepatitis B vaccine response in Guangxi population. HLA-DRB1 * 07 gene may be the cause of no / weak response to hepatitis B vaccine in Guangxi Zhuang and Han nationality.