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目的:研究不同采收期苍耳草的指纹图谱,建立稳定、可重复的苍耳草质量控制手段。方法:运用HPLC色谱技术建立不同采收期不同产地苍耳草的指纹图谱。结果:通过比较14批苍耳草和2批苍耳子药材样品HPLC图谱,建立其对照指纹图谱,并以共有模式为参照,通过《中药指纹图谱相似度计算软件》计算,得到色谱指纹图谱模拟图谱及相似度计算结果。除1号、15号和16号与其他批次样品相似度不高外,其他批次相似度较大,但是其主要成分在含量方面存在一定的差异,与药材采自不同采收期有关。结论:本文建立了苍耳草的HPLC指纹图谱,为苍耳草质量标准的建立提供了试验依据。
Objective: To study the fingerprints of Koraiensis at different harvesting stages and to establish a stable and reproducible method of quality control of Kom. Methods: The HPLC fingerprint was used to establish the fingerprints of Korax japonicus in different harvesting periods. Results: The HPLC fingerprints of 14 samples of Xanthium and 2 bats of Xanthium sibiricus were compared and their control fingerprints were established. Based on the common pattern, the HPLC fingerprinting method was used to calculate the similarity of fingerprints of Chinese herbs. Atlas and similarity calculation results. Except for Nos. 1, 15 and 16, the similarities with other batches were not high, but the similarity of other batches was quite large. However, there were some discrepancies in the content of the main constituents, which was related to the recovery of medicinal materials from different harvesting periods. Conclusion: This article established the HPLC fingerprint of Koraria japonica, which provided the experimental basis for establishing the quality standard of Kom.