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为了研究阴离子表面活性剂对鱼类的急性毒性效应,设计了不同浓度的十二烷基硫酸钠(SDS)溶液对草鱼进行暴露实验并测定其体内各组织SOD活性。急性毒性测试表明,SDS对草鱼的48h、96hLC50分别为11．8mg／L和5．2mg／L。低浓度的SDS(小于0．2mg/L)暴露对草鱼的超氧化物歧化酶(SOD)活性有极显著诱导作用,但随着SDS浓度升高和暴露时间延长,草鱼各组织SOD活性均呈明显的下降趋势,提示SDS暴露所引起的酶活性变化与暴露浓度和暴露时间有一定的相关性。这些结果显示,阴离子表面活性剂SDS对草鱼抗氧化酶有毒性效应,SOD活性变化可以作为评价环境中有毒污染物的生物敏感性指标。
In order to study the acute toxic effects of anionic surfactants on fish, different concentrations of sodium dodecyl sulfate (SDS) solution were designed to test the exposure of grass carp and the activity of SOD in different tissues. Acute toxicity tests showed that the 48h, 96hLC50 values of SDS for grass carp were 11.8mg / L and 5.2mg / L, respectively. Exposure to low concentrations of SDS (less than 0.2 mg / L) significantly induced the activity of superoxide dismutase (SOD) in grass carp. However, with the increase of SDS concentration and exposure time, the activities of SOD in the tissues of grass carp Significant downward trend, suggesting that changes in enzyme activity caused by exposure to SDS exposure time and exposure to a certain degree of correlation. These results show that the anionic surfactant SDS has a toxic effect on grass carp antioxidant enzymes, and the change of SOD activity can be used as a bio-sensitivity index to evaluate toxic pollutants in the environment.