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目的探讨肠型脂肪酸结合蛋白(FABP)2基因Ala54Thr多态性与非酒精性脂肪肝的关系。方法以聚合酶链反应(PCR)和限制性片段长度多态性(RFLP)方法,检测了234例非酒精性脂肪肝患者和476例正常对照者的FABP2基因Ala54Thr多态性,同时按照流行病学方法设计调查表进行问卷调查。结果FABP2基因型Ala54Ala、Ala54Thr和Thr54Thr在病例组中分别为39.7%、46.6%和13.7%,在对照组中分别为48.5%、43.1%和8.4%,差异有显著性(P<0.05)。病例组Thr等位基因频率(36.97%)明显高于对照组(29.94%)(P=0.01)。多因素Logistic回归分析显示,在调整混杂因素影响后,Thr54Thr基因型仍是非酒精性脂肪肝发病的独立危险因素,OR值为1.97,95%CI为1.18～3.35。结论FABP2基因Ala54Thr突变可能是非酒精性脂肪肝发病的遗传易感因素。
Objective To investigate the relationship between Ala54Thr polymorphism of FABP2 gene and nonalcoholic fatty liver disease. Methods The polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to detect Ala54Thr polymorphism of FABP2 gene in 234 patients with non-alcoholic fatty liver disease and 476 healthy controls. According to the epidemiology Study method design questionnaire survey. Results The FAGP2 genotypes Ala54Ala, Ala54Thr and Thr54Thr were 39.7%, 46.6% and 13.7% in the case group and 48.5%, 43.1% and 8.4% in the control group, respectively. The difference was significant (P <0.05). Thr allele frequency (36.97%) in case group was significantly higher than that in control group (29.94%) (P = 0.01). Multivariate Logistic regression analysis showed that Thr54Thr genotype was still an independent risk factor for nonalcoholic fatty liver disease after adjustment for confounding factors, OR was 1.97 and 95% CI was 1.18-3.35. Conclusion The mutation Ala54Thr of FABP2 gene may be the genetic predisposing factor for the non-alcoholic fatty liver disease.