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目的:应用Taqman qPCR技术检测CD147/basigin剪接变异体在人上皮性卵巢癌组织与正常卵巢组织中的表达差异。方法:运用半定量RT-PCR技术检测CD147/basigin剪接变异体在上皮性卵巢癌细胞系中的表达;Taqman qPCR检测CD147/basigin剪接变异体在人上皮性卵巢癌细胞系中的表达分布;进一步通过收集32例上皮性卵巢癌组织与26例正常卵巢组织,提取组织RNA,反转录cDNA,Taqman qPCR检测CD147/basigin剪接变异体mRNA在上皮性卵巢癌组织与正常卵巢组织中的表达差异。结果:半定量RT-PCR结果显示basigin-2,basigin-3和basigin-4在上皮性卵巢癌细胞系中均有表达,主要以basigin-2为主;Taqman qPCR检测到三种剪接变异体在不同卵巢癌细胞系中表达不同,basigin-2在卵巢癌细胞系中较basigin-3,basigin-4表达较高,basigin-4较basigin-3略高;Basigin-2剪接变异体在高转移Ho-8910pm细胞中表达较高,在低转移HO-8910细胞中表达较低。组织Taqman qPCR检测basigin-2和basigin-4在上皮性卵巢癌组织中的表达水平显著高于正常卵巢组织(P值分别为<0.0001和0.0261),basigin-3的表达水平略有升高(P=0.2616),但无统计学意义。结论:三种剪接变异体在卵巢癌组织中较正常卵巢组织表达上调。CD147/basigin-2在高转移卵巢癌细胞系HO-8910pm中高表达,在低转移卵巢癌细胞系HO-8910中低表达,且表达强度与上皮性卵巢癌的转移相关;探讨CD147/basigin-2在上皮性卵巢癌中的高表达,为卵巢癌的进一步治疗开辟一新途径。
Objective: To detect the difference of CD147 / basigin splicing variants in human epithelial ovarian cancer tissues and normal ovarian tissues using Taqman qPCR technique. Methods: The expression of CD147 / basigin splicing variants in epithelial ovarian cancer cell lines was detected by semi-quantitative RT-PCR. The expression and distribution of CD147 / basigin splicing variants in human epithelial ovarian cancer cell lines were detected by Taqman qPCR. The expression of CD147 / basigin splicing variant mRNA in epithelial ovarian cancer tissue and normal ovarian tissue was detected by collecting tissue RNA, reverse transcriptase cDNA and Taqman qPCR in 32 cases of epithelial ovarian cancer and 26 cases of normal ovarian tissue. Results: The results of semi-quantitative RT-PCR showed that basigin-2, basigin-3 and basigin-4 were all expressed in epithelial ovarian cancer cell lines, and mainly basigin-2. The three splice variants were detected by Taqman qPCR The expression of basigin-2 in different ovarian cancer cell lines was higher than that of basigin-3 and basigin-4 in ovarian cancer cell lines, while basigin-4 was slightly higher than that of basigin-3. -8910pm cells, and lower in low-metastatic HO-8910 cells. The expression of basigin-2 and basigin-4 in epithelial ovarian cancer was significantly higher than that in normal ovarian tissue (P <0.0001 and 0.0261, respectively) and basigin-3 slightly increased (P = 0.2616), but not statistically significant. Conclusion: The three splice variants are up-regulated in ovarian cancer tissues compared with normal ovarian tissues. CD147 / basigin-2 is overexpressed in highly metastatic ovarian cancer cell line HO-8910pm and is low expressed in low metastatic ovarian cancer cell line HO-8910, and its expression intensity is correlated with the metastasis of epithelial ovarian cancer. CD147 / basigin-2 The high expression in epithelial ovarian cancer opens up a new way for the further treatment of ovarian cancer.