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目的：研究内毒素对人肾小球内皮细胞（ＨＧＶＥＣ）活力的影响，探讨内毒素在烧伤早期损害中的作用。方法：分离培养人肾小球内皮细胞，把细胞分为正常培养组，内毒素浓度梯度组，内毒素时间梯度组，无血清内毒素作用ＨＧＶＥＣ１２ｈ组，另设烧伤血清时间梯度组为阳性对照。用ＭＴＴ法测定经内毒素和烧伤血清刺激后ＨＧＶＥＣ活力变化。结果：１μｇ／ｍｌ以上内毒素即可引起ＨＧＶＥＣ活力降低（Ｐ＜０．０１），１μｇ／ｍｌＬＰＳ时间梯度组细胞活力呈进行性下降，并在１２ｈ差异非常显著（与对照组比较）。在有血清和无血清条件下１μｇ／ｍｌＬＰＳ分别作用ＨＧＶＥＣ１２ｈ，细胞活力下降差别不显著。烧伤血清刺激组细胞活力６ｈ即出现明显降低，时间早于内毒素组，１μｇ／ｍｌ内毒素１２ｈ才显著降低，烧伤血清组降低程度大于内毒素组，两组间在３ｈ和６ｈ差异均显著。形态学观察，ＬＰＳ和烧伤血清作用ＨＧＶＥＣ可使其细胞核脱失。结论：内毒素和烧伤血清均可损伤ＨＧＶＥＣ，使其活力降低。ＬＰＳ对ＨＧＶＥＣ的损伤程度与ＬＰＳ浓度和作用时间呈正相关。内毒素对ＨＧＶＥＣ的损伤不依赖于血清参与。
Objective: To study the effect of endotoxin on the activity of human glomerular endothelial cells (HGVEC) and to explore the role of endotoxin in the early injury of burn. Methods: Human glomerular endothelial cells were isolated and cultured. The cells were divided into normal culture group, endotoxin concentration gradient group, endotoxin time gradient group, serum-free endotoxin-treated HGVEC 12h group, and burn serum time gradient group as positive control. The activity of HGVEC after stimulation with endotoxin and burn serum was measured by MTT assay. Results: The viability of HGVEC was significantly decreased by 1μg / ml of endotoxin (P <0.01). The viability of HGVEC decreased progressively in 1μg / ml LPS time gradient, and the difference was significant at 12h (compared with control group). HGVEC at 1μg / ml LPS treated with serum and serum-free serum for 12h showed no significant difference in cell viability. The cell viability in burn serum stimulated group decreased significantly at 6h, and the time was earlier than that in endotoxin group. The level of 1μg / ml endotoxin was significantly decreased 12h after burn injury, and the level of burn serum was lower than that of endotoxin group. The difference was significant at 3h and 6h. Morphological observation, LPS and burn serum HGVEC can make its nucleus lose. Conclusion: Both endotoxin and burn serum can damage HGVEC and reduce their vitality. The degree of damage to HGVEC by LPS was positively correlated with LPS concentration and duration of action. Endotoxin damage to HGVEC does not depend on serum involvement.