Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

• 作 者: Guanglin PENG Xiuchen XIN Yuanyuan CHE Zhifen ZHANG Shuyi SHI
• 机 构: Yantai Institute,China Agricultural University,
• 出 处: Agricultural Biotechnology
• 发表日期: 2012年03期
• 关键词: hybrida Hemerocallis Tender callus seedling dwarf panicle clustered aimed steril

[Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of Hemerocallis hybrida.[Method] Tender pedicels of dwarf H.hybrida were used as experimental materials to select callus induction,subculture and rooting media for rapid propagation of H.hybrida.[Result] MS+2.0-3.0 mg/L 6-BA+0.2-0.3 mg/L NAA,MS+1.0-2.0 mg/L 6-BA+0.1-0.2 mg/L NAA,MS and 1/2MS+0.2 mg/L NAA were the appropriate medium for callus induction,subculture and rooting,respectively.[Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H.hybrida,which provide technical reference for industrialized production of H.hybrida.

[Objective] This study aims to develop tissue culture and rapid propagation methods for pedicels of Hemerocallis hybrida. [Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to select callus induction, subculture and rooting media for rapid propagation of H. [ [Result] MS + 2.0-3.0 mg / L 6-BA + 0.2-0.3 mg / L NAA, MS + 1.0-2.0 mg / L 6-BA + 0.1-0.2 mg / L NAA, MS and 1/2 MS +0.2 mg / L NAA were the appropriate medium for callus induction, subculture and rooting, respectively. [Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technical reference for industrialized production of H.hybrida.