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目的观察密骨胶囊对自然衰老雄性大鼠血清骨碱性磷酸酶(BALP)和股骨生物力学性能的影响。方法取12月龄SPF级SD雄性大鼠110只,随机分为2组(空白对照组、密骨胶囊组),每组55只。自鼠龄12月龄始,分别给予0.9%NaCl溶液、密骨胶囊灌胃。在鼠龄14、16、18、20、22月龄时,每组各取10只大鼠,腹主动脉取血,分离血清,检测BALP;取右股骨,双能X线(DEXA)检测骨密度(BMD)后,采用三点弯曲法检测股骨生物力学指标(最大载荷、屈服载荷、弹性模量、能量吸收)。结果在14~22月龄阶段,与空白对照组比较,密骨胶囊可维持股骨BMD、弹性模量、屈服载荷和能量吸收;上调血清BALP浓度(P<0.01)。结论密骨胶囊抗骨质疏松作用可能是通过其促进骨形成,维持骨密度和骨生物力学性能而实现的。
Objective To observe the effects of Miaogu capsule on the biomechanical properties of serum bone alkaline phosphatase (BALP) and femur in natural aging male rats. Methods A total of 110 SPF SD male rats aged 12 months were randomly divided into 2 groups (blank control group and Miaogu capsule group), 55 in each group. From the age of 12 months of age, 0.9% NaCl solution and Migu capsule were given respectively. At the age of 14, 16, 18, 20, and 22 months of age, 10 rats in each group were taken from the abdominal aorta and serum was collected for the detection of BALP. The right femur and DEXA After BMD, biomechanical parameters (maximum load, yield load, elastic modulus and energy absorption) of the femur were measured by three-point bending method. Results Compared with the blank control group, Miangu capsule could maintain BMD, elastic modulus, yield load and energy absorption of the femur in 14 ~ 22 months of age, and increase serum BALP concentration (P <0.01). Conclusion The anti-osteoporosis effect of Miangu capsule may be achieved through its promotion of bone formation, maintenance of bone mineral density and bone biomechanical properties.