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已经公认细菌粘附于粘膜表面(包括泌尿道),在许多细菌建立感染上是重要的一步。阻止粘附可免于感染的可能性促进了粘附机理和粘附抑制物的研究。在寻找细菌粘附到泌尿道上皮细胞的抑制物尝试中,作者用了5种人免疫血清球蛋白(HISG)商品制剂和埃希氏大肠杆菌07KL、埃希氏大肠杆菌K13、奇异变形杆菌、肺炎杆菌、阴沟肠杆菌与大鼠膀胱上皮细胞进行了试验。方法是将从大鼠膀胱刮取的上皮细胞悬于pH7.4的磷酸盐缓冲液(PBS)稀释为1:20的HISG中,将等体积PBS洗过的细菌(10~9个/ml)加
It has been recognized that bacterial adhesion to mucosal surfaces, including the urinary tract, is an important step in the establishment of infection by many bacteria. The possibility of blocking adhesion from infection has facilitated the study of adhesion mechanisms and adhesion inhibitors. In an attempt to find inhibitors of bacterial adhesion to urothelial cells, the authors used five human immunoglobulin (HISG) commercial preparations and Escherichia coli 07KL, Escherichia coli K13, Proteus mirabilis, Klebsiella pneumoniae, Enterobacter cloacae and rat bladder epithelial cells were tested. The method is to suspend the epithelial cells scraped from the rat bladder in HISG diluted 1:40 in phosphate buffered saline (PBS) at pH 7.4, wash the bacteria (10-9 cells / ml) washed with an equal volume of PBS, plus