Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiati

• 作 者: Jie Du Xiaoqing Gao Li Deng Nengbin Chang Huailin Xiong Yu Zheng
• 机 构: Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,D
• 出 处: Neural Regeneration Research
• 发表日期: 2014年01期
• 关键词: neurotrophic factor marrow mesenchymal stem cells glial cell bone marrow epiderm

Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor.Cell viability,microtubule-associated protein 2-positive cell ratio,and the expression levels of glial cell line-derived neurotrophic factor,nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells.Furthermore,microtubule-associated protein 2,glial cell line-derived neurotrophic factor,nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells.These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells,and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor,nerve growth factor and growth-associated protein-43.

Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor / bone marrow mesenchymal stem cells compared with empty virus plasmid- transfected bone marrow mesenchymal stem cells. Frthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were higher than glial cell line-derived neurotrophic factor / bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mese These results suggest that glial cell line-derived neurotrophic factor / bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.