云烟87烤烟品种突变株的离体快繁技术

• 作 者: 吴艺飞 张战泓 周晓波 巢进 田茂成 田峰 朱三荣 欧阳娴 白占兵
• 机 构: 湖南省蔬菜研究所,湖南省蔬菜工程技术研究中心,湖南省烟草公司湘西自治州公司,
• 出 处: 中国烟草学报
• 发表日期: 2014年04期
• 关键词: 烤烟 突变株 离体快繁

为探讨特定条件下离体快繁对一些重要材料的优良性状进行迅速固定和繁殖的技术。以云烟87烤烟品种突变巨型株的休眠芽为试材,对其进行了打破休眠促使萌芽、愈伤组织诱导、丛生芽增殖和生根培养的研究。结果表明:打破休眠促使其萌芽的最佳培养基配方为MS+2.0 mg/L 6-BA+1.0 mg/L NAA;诱导产生愈伤组织效果最佳的培养基配方为MS+3.0 mg/L 6-BA+0.3 mg/L NAA;丛生芽增殖效果最佳的培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA;MS+0.6 mg/L NAA对根系的生长最为有利。

In order to explore the specific conditions of in vitro propagation of some important traits of fine traits of rapid fixation and propagation of technology. The dormant buds of giant tobacco mutant with flue-cured tobacco variety Yunyan 87 were used as materials to break the dormancy and promote budding, callus induction, proliferation and rooting of clustered buds. The results showed that the best medium for breaking dormancy was MS + 2.0 mg / L 6-BA + 1.0 mg / L NAA. The medium with the best callus induction was MS + 3.0 mg / L 6-BA +0.3 mg / L NAA. The best medium for the proliferation of clustered buds was MS + 1.0 mg / L 6-BA + 0.3 mg / L NAA. MS + 0.6 mg / L NAA was the most favorable for root growth.