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目的:观察丙泊酚对体外培养人肝癌HepG2细胞周期及凋亡的影响。方法:用不同浓度(30、60、120μg/mL)的丙泊酚处理HepG2细胞,分为P30、P60组及P120组,同时设对照组及溶剂脂肪乳组。48h后,TUNEL法及流式细胞仪检测HepG2细胞的凋亡;流式仪检测HepG2细胞周期。结果:TUNEL法显示各组HepG2细胞凋亡率(AI)为:对照组(1.80±0.23)%;脂肪乳组(1.90±0.20)%;P30组(2.02±0.31)%;P60组(2.10±0.28)%;P120组(2.30±0.36)%。与对照组比较,其他各组AI无统计学差异(P>0.05)。流式细胞图显示各组HepG2细胞的AI为:对照组0.22%,脂肪乳组0.61%,P30组2.15%,P60组2.12%,P120组1.91%。与对照组比较,P30、P60、P120组及脂肪乳组的细胞凋亡差异无统计学意义(P>0.05)。细胞周期图示:P30、P60、P120组及脂肪乳组细胞在G1、G2/M、S期的分布比例无统计学差异(P>0.05)。结论:30～120μg/mL的丙泊酚对HepG2细胞的细胞周期及凋亡无影响。
Objective: To observe the effect of propofol on the cell cycle and apoptosis of HepG2 cells cultured in vitro. Methods: HepG2 cells were treated with propofol of different concentrations (30, 60, 120μg / mL) and divided into P30, P60 and P120 groups. At the same time, control group and solvent fat emulsion group were established. After 48h, the apoptosis of HepG2 cells was detected by TUNEL and flow cytometry. The HepG2 cell cycle was detected by flow cytometer. Results: The apoptotic rate (AI) of HepG2 cells in each group was significantly higher than that in control group (1.80 ± 0.23)% by TUNEL method, 1.90 ± 0.20% in fat emulsion group (2.02 ± 0.31)%, P10 group 0.28)%; P120 group (2.30 ± 0.36)%. Compared with the control group, there was no significant difference in other groups (P> 0.05). Flow cytometry showed that the AI of HepG2 cells in each group was 0.22% in control group, 0.61% in fat emulsion group, 2.15% in P30 group, 2.12% in P60 group and 1.91% in P120 group. Compared with the control group, the apoptosis of P30, P60, P120 group and fat emulsion group showed no significant difference (P> 0.05). Cell cycle graph: P30, P60, P120 group and fat emulsion cells in the G1, G2 / M, S phase distribution was no significant difference (P> 0.05). Conclusion: Propofol 30 ~ 120μg / mL has no effect on cell cycle and apoptosis of HepG2 cells.