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目的观察不同频率和声压级水平(强度)的次声作用对大鼠胃黏膜生长抑素、P物质含量的影响,探讨次声对胃肠系统的作用机制。方法将140只大鼠随机分成4组,即对照组、8 Hz-90 dB组、8 Hz- 130 dB组及16 Hz-130 dB组,每组各35只。各实验组大鼠按分组时规定分别暴露于不同频率和强度的次声舱内(2 h/d),对照组大鼠则于相同时间内置于次声舱内(2 h/d),期间无次声干预。每组于次声作用1,7,14,21及28 d时各随机取出7只进行组织学准备,采用放射免疫法测定胃黏膜生长抑素(SS)、P物质(SP)含量。结果①8 Hz-90 dB次声作用14 d和21 d后,胃黏膜SS含量较对照组显著升高(P<0.05);作用1 d、7 d及28 d时与对照组比较,差异则无统计学意义(P>0.05)。8 Hz-130 dB次声作用7 d、14 d、21 d及28 d后胃黏膜SS含量均明显高于对照组(P<0.01),其中以第14天时最为显著;作用1 d时与对照组比较,差异无统计学意义(P>0.05)。16 Hz-130 dB次声作用1 d、7 d及14 d后胃黏膜SS含量较对照组明显升高(P<0.05或0.01),其中以第7天时最为显著;作用21 d及28 d时与对照组比较,差异无统计学意义(P>0.05)。8 Hz- 130 dB次声作用7 d、14 d及21 d后胃黏膜SS含量明显高于8 Hz-90 dB组(P<0.05或0.01),作用14 d、21 d及28 d后显著高于16 Hz-130 dB组(P<0.05或0.01)。②8 Hz,90 dB和16 Hz,130 dB次声作用1 d、7 d、14 d、21 d及28 d后胃黏膜SP含量与对照组比较,差异均无统计学意义(P>0.05)。8 Hz-130 dB次声作用14 d和21 d后胃黏膜SP含量明显高于对照组(P<0.05);作用1 d,7 d及28 d后与对照组比较,差异无统计学意义(P>0.05)。8 Hz,130 dB次声作用1 d、7 d、14 d、21 d及28 d后胃黏膜SP含量与8 Hz,90 dB和16 Hz,130 dB组比较,差异均无统计学意义(P>0.05)。结论①次声作用可诱发实验大鼠胃黏膜SS、SP含量升高,其升高的幅度与次声作用的频率、强度及时间有关。②大鼠经次声作用多次后可产生一定的适应性。
Objective To observe the effect of infrasound of different frequency and sound pressure level (intensity) on the content of somatostatin and substance P in rat gastric mucosa and to explore the mechanism of infrasound on the gastrointestinal system. Methods 140 rats were randomly divided into 4 groups: control group, 8 Hz-90 dB group, 8 Hz-130 dB group and 16 Hz-130 dB group, 35 rats in each group. The rats in each experimental group were exposed to infrasound at different frequencies and intensities for 2 h / d respectively according to the time of grouping while the rats in control group were placed in infrasound for 2 h / d at the same time, No inflectional intervention. Seven rats in each group were randomly selected for histological preparation at 1, 7, 14, 21 and 28 days after infrasound. The contents of somatostatin (SS) and substance P in gastric mucosa were determined by radioimmunoassay. Results ① After 14 days and 21 days of 8 Hz-90 dB infiltration, the content of SS in gastric mucosa was significantly higher than that in the control group (P <0.05). On the 1st, 7th and 28th days, the differences were not found Statistical significance (P> 0.05). The gastric mucosal SS content of 8 Hz-130 dB infrasound at 7 d, 14 d, 21 d and 28 d were significantly higher than that of the control group (P <0.01), of which the most significant was on the 14th day. There was no significant difference between the two groups (P> 0.05). At 1 d, 7 d and 14 d, the SS content of gastric mucosa in 16 Hz-130 dB group was significantly higher than that in control group (P <0.05 or 0.01), of which the most significant was on the 7th day. At 21 d and 28 d Compared with the control group, the difference was not statistically significant (P> 0.05). The gastric mucosal SS content was significantly higher in 8 Hz-130 dB group than that in 8 Hz-90 dB group (P <0.05 or 0.01) after 7 d, 14 d and 21 d of 8 Hz-130 dB, and significantly higher after 14 d, 21 d and 28 d At 16 Hz-130 dB (P <0.05 or 0.01). ② There was no significant difference in gastric mucosal SP content at 8 Hz, 90 dB, 16 Hz and 130 dB infrasound after 1 d, 7 d, 14 d, 21 d and 28 d compared with the control group (P> 0.05). The gastric mucosal SP content of 8 Hz-130 dB infrasound for 14 d and 21 d was significantly higher than that of the control group (P <0.05). There was no significant difference in the SP content between the control group and the 1 d, 7 d and 28 d P> 0.05). The gastric mucosal SP content at 1, 7, 14, 21, and 28 days after 8 Hz and 130 dB had no significant difference compared with those at 8 Hz, 90 dB, 16 Hz and 130 dB (P > 0.05). Conclusions ① Infrasound can induce gastric mucosal SS and SP in experimental rats to increase, and its increasing amplitude is related to the frequency, intensity and time of infrasound. ② rats after repeated infrasound can produce some adaptability.