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已知花生四烯酸可被前列腺素G /H合成酶氧化成前列腺素,或被脂氧合酶氧化成白三烯,前者如PGE_2和PGI_2等能抑制组织胺、五肽胃泌素及迷走神经刺激的胃酸分泌和增加胃粘膜血流,而后者则可促进炎症和延缓溃疡的愈合。本文测定了应激性溃疡时上述两酸的活力以及前列腺素和白三烯合成的变化。材料和方法:取Holtzman鼠20只,分成两组,每组10只,实验组鼠禁食24小时后给4℃冷应激4小时,对照组鼠不给刺激。处死动物后,取出胃,沿小弯切开,见实验组胃内有凝血块,而对照组胃内无凝血块。分别取胃内病变和非溃疡区的粘膜组织,切碎,秤150mg组织,用盐水缓冲液冲洗,在1.3mM花生四烯酸钠溶液中培养10分钟,该溶液含有非离子化去污剂(Pcl-oxamer 188)、NaCl、KCl、K_3PO_4、谷胱甘肽、血红素、MgSO_4和Hepes,其pH为8。提炼出花生四烯酸盐氧
It is known that arachidonic acid can be oxidized to prostaglandins by prostaglandin G / H synthase or oxidized by lipoxygenase to leukotrienes. The former, such as PGE 2 and PGI 2, can inhibit histamine, pentagastrin and vagus nerve Stimulated gastric acid secretion and increased gastric mucosal blood flow, while the latter can promote inflammation and delay ulcer healing. This article measured stress ulcer when the two acid activity and prostaglandin and leukotriene synthesis changes. MATERIALS AND METHODS: Twenty Holtzman rats were divided into two groups with 10 rats in each group. The rats in the experimental group were given 4-hour cold stress for 4 hours after fasting, and the rats in the control group did not receive stimulation. After the animals were sacrificed, the stomach was removed and incised along a small curve. The experimental group had a clot in the stomach, while the control group had no clot in the stomach. The lesions in the stomach and the mucosa in the non-ulcer area were separately harvested, shredded, and weighed 150 mg and washed with saline buffer and incubated for 10 minutes in a 1.3 mM sodium arachidonate solution containing a non-ionic detergent Pcl-oxamer 188), NaCl, KCl, K_3PO_4, glutathione, heme, MgSO_4 and Hepes. Extract arachidonate oxygen