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目的:建立大脑中动脉闭塞(MCAO)大鼠模型,观察化瘀解毒法对MCAO大鼠血浆TAT、F1+2、v WF、D-二聚体含量的影响。方法:雄性SD大鼠56只,随机分为假手术组、模型组、阿加曲班对照组、活血化瘀组、清热解毒组、化瘀解毒法组及PDTC组。线栓法制备大鼠左侧大脑中动脉闭塞模型,分别于术前1 h和术后12 h各给予相应药物1次,术后24h取标本用于检测。结果:(1)造模后各组TAT、F1+2水平显著升高;各治疗组TAT、F1+2水平较模型组明显降低(均P<0.05);各治疗组之间比较,TAT的含量以PDTC组最高,其次化瘀解毒法组和清热解毒组,阿加曲班组和活血化瘀组最低;F1+2含量阿加曲班组较PDTC组、化瘀解毒法组、活血化瘀组均低,化瘀解毒法组较PDTC组低(均P<0.05)。(2)造模后D-二聚体、v WF的水平显著增高;与模型组相比,阿加曲班组D-二聚体的水平降低(P<0.05),清热解毒组D-二聚体的含量升高(均P<0.05),活血化瘀组、化瘀解毒法组、PDTC组与模型组之间差异无统计学意义;各治疗组v WF的含量较模型组均明显降低(P<0.05);各治疗组之间比较,活血化瘀组v WF的含量较PDTC组、清热解毒组均高(P<0.05);活血化瘀组、阿加曲班组、化瘀解毒法组之间差异无统计学意义(P>0.05)。(3)活血化瘀法和清热解毒法配伍存在协同作用,活血化瘀中药和清热解毒中药单用均有降低血浆TAT、F1+2、v WF水平的作用,同用时交互作用有统计学意义(P<0.05)。结论:MCAO模型大鼠血液呈现高凝状态,表现为凝血功能和纤维蛋白溶解系统功能亢进、血小板活化增强,活血化瘀法、清热解毒法以及化瘀解毒法对此均有不同程度的改善作用,其中活血化瘀法主要侧重凝血和纤维蛋白溶解系统功能的改善,清热解毒法主要侧重血小板功能和血管内皮功能的改善,而化瘀解毒法对两者均起作用,且具有协同效应。
OBJECTIVE: To establish a rat model of middle cerebral artery occlusion (MCAO) and observe the effect of activating blood stasis and detoxification on plasma TAT, F1 + 2, v WF and D-dimer in MCAO rats. Methods: Fifty-six male SD rats were randomly divided into sham operation group, model group, argatroban control group, Huoxuehuayu group, Qingrejiedu group, Huayu detoxification group and PDTC group. The left middle cerebral artery occlusion model was prepared by thread plug method, and the corresponding drugs were given at 1 h before surgery and 12 h after surgery respectively. Results: (1) The levels of TAT and F1 + 2 in each group were significantly increased after modeling. The levels of TAT and F1 + 2 in each treatment group were significantly lower than those in model group (all P <0.05) The highest in PDTC group, followed by Huayu detoxification group and Qingrejiedu group, argatroban group and Huoxuehuayu group, the lowest in F1 + 2 group compared with PDTC group, Huayu detoxification group and Huoxuehuayu group Both were lower, blood stasis detoxification group than the PDTC group (all P <0.05). (2) The levels of D-dimer and v WF in model group were significantly higher than those in model group (P <0.05), and D-dimer in detoxification group (P <0.05). There was no significant difference in the content of vWF between the treatment group and the model group (P> 0.05) P <0.05). Compared with PDTC group and Qingrejiedu group, the content of vWF in blood-activating-blood-stasis group was significantly higher than that in the other two groups (P <0.05) The difference was not statistically significant (P> 0.05). (3) There is a synergistic effect between the methods of promoting blood circulation to remove blood stasis and the compatibility of clearing away heat and toxic materials. Both TCM of promoting blood circulation and removing blood stasis and clearing away heat and toxic materials of traditional Chinese medicine alone have the effect of lowering plasma TAT, F1 + 2 and vWF levels, with significant statistical significance (P <0.05). CONCLUSION: The blood of rats with MCAO shows hypercoagulable state, showing coagulation function and hypertrophy of fibrinolytic system, increased platelet activation, promoting blood circulation and removing blood stasis method, clearing away heat and toxic materials, and removing blood stasis and detoxification to varying degrees , Of which the method of promoting blood circulation and removing blood stasis mainly focuses on the improvement of coagulation and fibrinolytic system functions. The method of clearing away heat and toxic materials mainly focuses on the improvement of platelet function and endothelial function, while the method of clearing away blood stasis and detoxification plays a role of both and has a synergistic effect.