论文部分内容阅读
R带是在染色体的纵向上显示明暗相间的带纹,它的特点是带型恰和Q带、G带相反,除Y染色体外,其余各对染色体的末端均为深带,故对揭示涉及染色体末端的缺失和易位特別有价值。近来我们对20余例白血病患者(其中ANLL10例,ALL4例,CGL8例)采用Dutrillaux等的方法结合本实验室的条件加以改良,制备骨髓细胞染色体R带获得成功。具体方法如下: 1.骨髓细胞的培养、收获、低渗和固定等均按常规进行。制成的细胞悬液保存于冰箱内至少1天以上,再取出滴片。每份标本制片9～12张,平放于滤纸上,室温下放置1小时。 2.取3只容量为50ml的立式染缸,每只缸内倒入Earle溶液各50ml,加盖后置水浴箱中,使水温升至87.5℃,然后每个染色缸内投入上述制片
The R band shows the light and dark bands in the longitudinal direction of the chromosome. It is characterized by the band type just like the Q band, and the G band is the opposite. Except for the Y chromosome, the ends of each pair of the chromosomes are deep bands. Therefore, Chromosomal deletions and translocations are particularly valuable. Recently more than 20 cases of leukemia patients (including ANLL10 cases, ALL4 cases, CGL8 cases) using Dutrillaux method combined with the laboratory conditions to be improved, the preparation of bone marrow cells chromosome R band was successful. Specific methods are as follows: 1. Bone marrow cell culture, harvest, hypotonic and fixed Dengjun routine. Prepared cell suspension stored in the refrigerator for at least 1 day, and then remove the tablets. Each specimen preparation 9 to 12, flat on the filter paper, at room temperature for 1 hour. 2. Take three 50ml capacity of vertical dye vat, each tank into the Earle solution of 50ml, stamped after setting water bath, the water temperature rose to 87.5 ℃, and then into each dye cylinder production