Selection,Expression and Purification of Human Abzyme Containing Selenium with Type Ⅰ Thyroxine Deio

• 作 者: LIN Feng LIU Lan-ying NI Jia-z
• 机 构: CollegeofLifeScience,KeyLaboratoryofRareEarthofChineseAcademy,CollegeofLifeScience
• 出 处: 高等学校化学研究：英文版
• 发表日期: 2011年4期
• 关键词: 甲状腺素脱碘酶 硒代半胱氨酸 抗体酶 纯化 分离 DNA序列分析 噬菌体展示 人类 Phage antibody library Humanization
• 基金项目: Supported by the Basic Science Research Fund of Jilin University China（No.200903259）

Single-chain fragment variable(ScFv) antibodies with the substrate binding sites were obtained by repetitive selections from a semi-synthetic phage display antibody library against two haptens——thyroxine(T4) and O-methyl-T4(O-CH3-T4).The positive phage cl