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目的 :探讨利用热休克蛋白 70 (HSP70 )基因转染技术保护人心肌细胞的可行性。方法 :用脂质体介导的基因转移技术 ,研究了外源性HSP70基因高表达对人心肌细胞的保护作用。结果 :转染的心肌细胞在正常温度 ( 37℃ )下 ,可高水平表达HSP70 ,模拟缺血实验后 ,心肌肌酸激酶 (CK MB)释放量为( 159.5± 2 4 .1)IU/L ,细胞生存率为 ( 33.78± 5.4 ) % ,其抗模拟缺血能力明显高于对照组 ,而与热休克预处理后 2 4小时组无显著差异。结论 :基因转染造成的HSP70高表达可保护人心肌细胞
Objective: To explore the feasibility of using heat shock protein 70 (HSP70) gene transfection technology to protect human cardiomyocytes. Methods: Liposome-mediated gene transfer technique was used to study the protective effect of exogenous HSP70 gene on human cardiomyocytes. Results: The transfected cardiomyocytes could express HSP70 at high temperature (37 ℃), and the release of CK MB after simulated ischemia was (159.5 ± 24.1) IU / L , The cell survival rate was (33.78 ± 5.4)%, its anti-ischemic ability was significantly higher than that of the control group, and 24 h after heat shock pretreatment group no significant difference. Conclusion: The high expression of HSP70 induced by gene transfection can protect human cardiomyocytes