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目的观察对转染CXCR4基因的骨髓间充质干细胞体外生物学行为的影响。方法将培养的骨髓间充质干细胞分为3组,GFP组、CXCR4~+组、CXCR4~-组,转染趋化因子受体CXCR4,并用免疫荧光细胞化学法、流式细胞仪法及Transwell小室细胞趋化实验,体外研究了CXCR4高表达及低表达对MSCs增殖、分化与迁移能力的影响。结果 CXCR4高表达及低表达均不影响MSCs的增殖能力,对其向肺组织分化的能力也没有影响。与GFP-MSCs组相比,CXCR4~+-MSCs组迁移的细胞数量明显升高,而CXCR4~--MSCs组迁移细胞数量差异无显著性。结论 CXCR4高表达及低表达不改变MSCs的增殖、分化能力,然而CXCR4高表达明显增强MSCs向炎症病灶的迁移能力。说明CXCR4高表达的MSCs移植入体后将会更快速、更大量地到达病变区域参与组织修复,明显增强疗效。
Objective To observe the effect of transfection of CXCR4 gene on the biological behavior of bone marrow mesenchymal stem cells in vitro. Methods BMSCs were divided into three groups: GFP group, CXCR4 ~ + group and CXCR4 ~ - group. The chemotactic CXCR4 cells were transfected with the chemokine receptor CXCR4. Immunofluorescence cytochemistry, flow cytometry and Transwell Cell chemotaxis assay was used to investigate the effects of CXCR4 overexpression and low expression on proliferation, differentiation and migration of MSCs in vitro. Results Both high expression of CXCR4 and low expression of CXCR4 did not affect the proliferation of MSCs and had no effect on their ability to differentiate into lung tissue. Compared with GFP-MSCs group, CXCR4 ~ + -MSCs group had a significant increase in the number of migrating cells, while CXCR4 ~ - MSCs group had no significant difference in number of migrating cells. Conclusion The high expression and low expression of CXCR4 do not change the proliferation and differentiation of MSCs. However, the high expression of CXCR4 significantly enhances the migration of MSCs to inflammatory lesions. The results showed that CXCR4-overexpressing MSCs could reach the lesion more rapidly and in greater quantities and participate in tissue repair after transplantation into the body, which markedly enhanced the curative effect.