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为了测定人体感染淋球菌后的局部体液免疫反应。我们从１５例无并发症的男性淋病患者的急性期和恢复期以及正常人对照中收集精液标本，经硫酸胺沉淀法纯化抗体后，用免疫印迹实验技术测定其对同一患者分离得到的淋球菌的外膜蛋白的抗原抗体反应。结果，精液中的抗体在急性期与淋球菌外膜蛋白抗原起反应的量多于恢复期，在同一标本ＩｇＡ型抗体量也多于ＩｇＧ型抗体。主要的反应抗原是ＰＩ、ＰＩ、４６０００～４８０００、１４０００～１６０００和８８０００～９００００。正常对照的１５份标本中仅２份对１４０００～１６０００抗原有反应。
In order to determine the human body after the infection of Neisseria gonorrhoeae local humoral immune response. We collected semen samples from acute and convalescent patients with gonorrhea in 15 non-complication cases and normal controls. Antibody was purified by ammonium sulfate precipitation method and then assayed by immunoblot assay for gonococcal isolates from the same patient Of the outer membrane protein antigen antibody reaction. As a result, antibodies in semen reacted more frequently with gonococcal outer membrane antigens in the acute phase than in convalescent ones, and more IgA-type antibodies were detected in the same sample than IgG-type antibodies. The major reactive antigens are PI, PI, 46000-48000, 14000-16000 and 88000-90000. Only two out of 15 samples of normal controls responded to 14000-16000 antigens.