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目的：探讨Ｂｃｌ－２蛋白在乳腺癌及良性乳腺病变中的表达，观察Ｂｃｌ－２蛋白与乳腺癌预后参数的关系。方法：采用免疫组化方法检测 ６９例浸润乳癌，４５例导管原位癌（ｄｕｃｔａｌｃａｒｃｉｎｏｍａ ｉｎ ｓｉｔｕ，ＤＣＩＳ），１５例正常乳腺组织，３８例乳腺良性增生病变中Ｂｃｌ－２蛋白表达。结果：Ｂｃｌ－２蛋白在浸润乳癌，ＢＣＩＳ，乳腺良性增生病变以及正常乳腺组织阳性率分别为６５．２％，７５．６％，１００％，１００％。Ｂｃｌ－２蛋白表达与乳腺癌好的预后参数有关，如肿瘤体积小，低的组织学分级，ＥＲ、ＰＲ阳性，坏死出现少（Ｐ＜０．０１），低ｐ５３蛋白表达（Ｐ＜０．０５），与腋淋巴结浸润没有关系（Ｐ＞０．０５）。大部分乳腺癌表现 Ｂｃｌ－２＋／ｐ５３－表达表型，多属于高分化和中分化病例。而正常乳腺组织，良性增生病变中均为 Ｂｃｌ－２＋／ｐ５３－表达表型。结论：①Ｂｃｌ－２蛋白在正常乳腺组织、良性增生病变的表达高于乳腺癌的表达。②Ｂｃｌ－２蛋白表达与好的乳腺癌预后因素有关。③Ｂｃｌ－２与ｐ５３两种基因蛋白负相关。
Objective: To investigate the expression of Bcl-2 protein in breast cancer and benign breast lesions and to observe the relationship between Bcl-2 protein and the prognosis of breast cancer. Methods: Immunohistochemistry was used to detect the expression of Bcl-2 protein in 69 cases of invasive breast cancer, 45 cases of ductal carcinoma in situ (DCIS), 15 cases of normal breast tissue and 38 cases of benign breast hyperplasia. Results: The positive rates of Bcl-2 protein in invasive breast cancer, BCIS, benign breast hyperplasia and normal breast tissues were 65.2%, 75.6%, 100% and 100%, respectively. The expression of Bcl-2 protein was correlated with the prognosis parameters of breast cancer, such as small tumor volume, low histological grade, positive ER and PR, less necrosis (P <0.01) and lower expression of p53 protein (P <0. 05), and axillary lymph node infiltration (P> 0.05). Most of the breast cancer Bcl-2 / p53 expression phenotype, mostly in well-differentiated and moderately differentiated cases. The normal breast tissue, benign hyperplasia lesions are Bcl-2 / p53-expressing phenotype. Conclusion: ① The expression of Bcl-2 protein in normal breast tissue and benign hyperplasia lesions is higher than that in breast cancer. ② Bcl-2 protein expression and prognosis of breast cancer are related factors. ③ Bcl-2 and p53 two gene protein negative correlation.