Authentication of Fungal Mycelium Isolated from Boletus appendiculatus Fruit Body Tissue Using RAPD

• 出 处: 食用菌学报
• 发表日期: 2007年01期
• 关键词: Authentication Boletus fungal fungi Amplification agarose sequencing genomic seq

RAPD molecular markers and ITS sequence comparisons were used to establish the relationship between Boletus appendiculatus fruit bodies collected from Qingyuan, Zhejiang Province, and mycelium isolated from the fruit body tissue. RAPD analysis of extracted genomic DNA revealed that B. appendiculatus fruit bodies (fb) and the isolated fungal mycelium (mb) formed a single cluster with a Dice coefficient value of 0.92. Comparison of ITS sequences from these samples showed that base sequences and fragment lengths were identical, thereby confirming that the fungal mycelium isolated from B. appendiculatus fruit bodies was the vegetative form of the mushroom. Our data suggest that RAPD fingerprinting combined with ITS sequence comparisons constitute an effective and feasible method for identifying mycelium isolated from ectomycorrhizal fungi.

RAPD analysis of extracted genomic DNA revealed that B. appendiculatus fruit bodies collected from Qingyuan, Zhejiang Province, and mycelium fruit bodies (fb) Comparison of ITS sequences from these samples showed that base sequences and fragment lengths were identical, thereby confirming that the fungal mycelium isolated from B. appendiculatus fruit bodies was the vegetative form of the mushroom. Our data suggest that RAPD fingerprinting combined with ITS sequence comparisons constitute an effective and feasible method for identifying mycelium isolated from ectomycorrhizal fungi.