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用ｐＧＥＸ载体系统体外构建了一种人精子膜蛋白片段（ＨＳＤⅡ）的重组表达质粒．未经ＩＰＴＧ诱导，该质粒表达的融合蛋白在ＤＨ５α中即有较高的本底表达量．若将带有ＬａｃⅠ基因的ｐＲＥＰ４质粒与重组表达质粒共转化ＤＨ５α菌，则可有效抑制融合蛋白的本底表达．
A recombinant expression plasmid of human sperm membrane protein fragment (HSDⅡ) was constructed with pGEX vector system in vitro. Without IPTG induction, the fusion protein expressed by this plasmid had a higher background expression in DH5α. If pREP4 plasmid with Lac Ⅰ gene was co-transformed into DH5α strain with recombinant expression plasmid, the background expression of fusion protein could be effectively inhibited.