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目的:建立孕马尿中主要结合雌激素的SPE-AgNO3-TLC定性方法。方法:采用大孔树脂固相萃取柱对孕马尿中结合雌激素富集净化,通过盐洗、碱洗除去部分杂质,乙醇进行洗脱,采用AgNO3-TLC鉴别孕马尿中3种主要结合雌激素。结果:薄层色谱中,供试品色谱与对照品色谱在相应位置显相同颜色的条带,条带清晰,分离效果好,阴性无干扰。结论:SPE-AgNO3-TLC适合孕马尿中主要结合雌激素的定性检测,对于孕马尿中结合雌激素品质评价具有重要参考价值。
OBJECTIVE: To establish a qualitative SPE-AgNO3-TLC method for the determination of the main conjugated estrogen in pregnant and urine. Methods: Macroporous resin solid phase extraction column was used to enrich and purify urinary conjugated estrogen in pregnant and mare urine. Some impurities and ethanol were removed by salt washing and alkaline washing. AgNO3-TLC was used to identify the three major bindings Estrogen. Results: In TLC, the chromatogram of the test sample and the reference substance showed the same color band at the corresponding position, the band was clear, the separation effect was good, and the negative was non-interference. Conclusion: SPE-AgNO3-TLC is suitable for the qualitative detection of the main conjugated estrogen in pregnant and mare urine. It has important reference value for evaluating the quality of combined estrogen in pregnant and urine.